An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles
An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles
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DOI：
10.1016/j.vaccine.2008.01.053
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发表时间：
2008-03-28
期刊：
影响因子：
5.5
通讯作者：
Mason, Hugh S.
中科院分区：
文献类型：
--
作者：
Santi, Luca;Batchelor, Lance;Mason, Hugh S.
来源于肠道病原体如诺瓦克病毒（NV）的病毒样颗粒（VLP）非常适合研究口服免疫。我们以前描述了稳定的转基因植物，积累重组NV样颗粒（rNV），口服免疫原性小鼠和人类。转基因方法具有世代时间长和水平低的缺点。抗原的积累。现在，我们克服了这些限制，一个有效的烟草花叶病毒（TMV）衍生的瞬时表达系统，利用烟草本塞姆氏叶。我们在感染后12天（dpi）以0.8mg/g叶产生了特性组装的rNV。口交。用100或250 μ g/剂量的部分纯化的rNV免疫CD 1小鼠引起全身和粘膜免疫应答。我们的结论是，植物病毒瞬时表达系统提供了一个强大的研究工具，以产生大量的rNV富集，浓缩的VLP制剂，口服免疫原性。(C)2008爱思唯尔有限公司保留所有权利。
Virus-like particles (VLPs) derived from enteric pathogens like Norwalk virus (NV) are well suited to study oral immunization. We previously described stable transgenic plants that accumulate recombinant NV-like particles (rNVs) that were orally immunogenic in mice and humans. The transgenic approach suffers from tong generation time and modest Level. of antigen accumulation. We now overcome these constraints with an efficient tobacco mosaic virus (TMV)-derived transient expression system using leaves of Nicotiana benthamiana. We produced property assembled rNV at 0.8 mg/g leaf 12 days post-infection (dpi). Oral. immunization of CD1 mice with 100 or 250 mu g/dose of partially purified rNV elicited systemic and mucosal immune responses. We conclude that the plant viral transient expression system provides a robust research toot to generate abundant quantities of rNV as enriched, concentrated VLP preparations that are orally immunogenic. (C) 2008 Elsevier Ltd. All rights reserved.