Axon terminals expressing vesicular glutamate transporter VGLUT1 or VGLUT2 within the trigeminal motor nucleus of the rat: Origins and distribution patterns
Axon terminals expressing vesicular glutamate transporter VGLUT1 or VGLUT2 within the trigeminal motor nucleus of the rat: Origins and distribution patterns
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DOI：
10.1002/cne.21894
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发表时间：
2009-02
影响因子：
2.5
通讯作者：
Y. Pang;S. Ge;KouichiC . Nakamura;Jinlian Li;K. Xiong;T. Kaneko;N. Mizuno
中科院分区：
文献类型：
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作者：
Y. Pang;S. Ge;KouichiC . Nakamura;Jinlian Li;K. Xiong;T. Kaneko;N. Mizuno
关于两种类型的谷氨酸能神经元（表达囊泡谷氨酸转运蛋白 VGLUT1 或 VGLUT2）在控制下颌运动中的重要性知之甚少。因此，我们检查了大鼠三叉神经运动核（Vm）内具有 VGLUT1 或 VGLUT2 免疫反应性的轴突末端的起源和分布。 Vm 分为背外侧区（Vm.dl；闭颌运动神经元池）和腹内侧区（Vm.vm；张颌运动神经元池）。 VGLUT1 免疫阳性末端仅在 Vm.dl 中可见，而 VGLUT2 免疫阳性末端分布在 Vm.dl 和 Vm.vm 中。运动根的横断消除了 Vm.dl 中几乎所有 VGLUT1 免疫阳性轴突，两个分裂中的 VGLUT2 免疫反应性没有变化，表明 VGLUT1 和 VGLUT2 免疫阳性轴突分别来自中脑三叉神经核的初级传入神经元和 Vm 的前运动神经元。原位杂交组织化学显示，在与报道的 Vm 前运动神经元池相对应的区域中，VGLUT2 神经元比 VGLUT1 神经元数量多得多。免疫荧光标记结合顺行束追踪的结果进一步表明，三叉神经感觉核、三叉上区和Vm腹侧网状区中带有VGLUT2的前运动神经元发送轴突末端接触三叉神经运动神经元，并且Vm腹侧网状区中一些表达VGLUT1的前运动神经元发送轴突末端至闭颌运动神经元。目前的结果表明，表达 VGLUT1 和 VGLUT2 的谷氨酸能神经元在控制下颌运动中所发挥的作用可能不同。 J.Comp。内罗尔。 512:595–612, 2009。© 2008 Wiley-Liss, Inc.
Little is known about the significance of the two types of glutamatergic neurons (those expressing vesicular glutamate transporter VGLUT1 or VGLUT2) in the control of jaw movements. We thus examined the origin and distribution of axon terminals with VGLUT1 or VGLUT2 immunoreactivity within the trigeminal motor nucleus (Vm) in the rat. The Vm was divided into the dorsolateral division (Vm.dl; jaw‐closing motoneuron pool) and the ventromedial division (Vm.vm; jaw‐opening motoneuron pool). VGLUT1‐immunopositive terminals were seen within the Vm.dl only, whereas VGLUT2‐immunopositive ones were distributed to both the Vm.dl and the Vm.vm. Transection of the motor root eliminated almost all VGLUT1‐immunopositive axons in the Vm.dl, with no changes of VGLUT2 immunoreactivity in the two divisions, indicating that the VGLUT1‐ and VGLUT2‐immunopositive axons came from primary afferents in the mesencephalic trigeminal nucleus and premotor neurons for the Vm, respectively. In situ hybridization histochemistry revealed that VGLUT2 neurons were much more numerous than VGLUT1 neurons in the regions corresponding to the reported premotoneuron pool for the Vm. The results of immunofluorescence labeling combined with anterograde tract tracing further indicated that premotor neurons with VGLUT2 in the trigeminal sensory nuclei, the supratrigeminal region, and the reticular region ventral to the Vm sent axon terminals contacting trigeminal motoneurons and that some of the VGLUT1‐expressing premotor neurons in the reticular region ventral to the Vm sent axon terminals to jaw‐closing motoneurons. The present results suggested that the roles played by glutamatergic neurons in controlling jaw movements might be different between VGLUT1‐ and VGLUT2‐expressing neurons. J. Comp. Neurol. 512:595–612, 2009. © 2008 Wiley‐Liss, Inc.