The Roles of Somatostatin-Expressing (GIN) and Fast-Spiking Inhibitory Interneurons in UP-DOWN States of Mouse Neocortex
The Roles of Somatostatin-Expressing (GIN) and Fast-Spiking Inhibitory Interneurons in UP-DOWN States of Mouse Neocortex
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DOI：
10.1152/jn.00206.2010
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发表时间：
2010-08-01
影响因子：
2.5
通讯作者：
Connors, Barry W.
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文献类型：
--
作者：
Fanselow, Erika E.;Connors, Barry W.
Fanselow EE，Connors BW。在小鼠新皮层的上向下状态下表达生长抑素（GIN）和快速刺激性抑制性神经元的作用。 J Neurophysiol 104：596-606，2010年。2010年6月10日首次出版； doi：10.1152/jn.00206.2010。新皮层包含多种类型的抑制性神经元，其特性表明它们可能在皮质回路中起不同的作用。通过在体外两个不同的网络状态（上下状态）中从三种细胞类型记录，我们能够量化不同网络状态期间这些细胞之间的点火特性差异。我们从常规刺激性（RS）兴奋性细胞和两种类型的抑制性神经元中记录，快速加速器（FS）神经元和GFP-（以及表达抑制性神经元）的GFP-（以及Somatostatin-），在小鼠中的2/3层中。体感新皮层。在上下验收期间，这些细胞之间的点火特性比较显示了几种模式。首先，在这些细胞类型中，只有杜松子酒细胞在下降状态下持续发射，而在向上状态下，所有三种细胞类型都很容易发射。其次，在整个状态的触发和动作电位的发作和分布因细胞类型而异，这表明FS UP-State发射优先发生在UP-State的开头附近，而RS元素的启动较慢以在在上升状态的整个过程中，上升状态的开始和杜松子酒的射击持续。最后，在上州的膜中，异质细胞类型之间的膜电势和尖峰相关性更为明显，并且对于在杜松子酒细胞上的RS突触中，在整个UPSTATE上都有变化。这些结果表明，随着UPSTATE的进展，FS和杜松子酒细胞之间存在劳动力分裂，并表明杜松子酒细胞在终止上可能很重要。
Fanselow EE, Connors BW. The roles of somatostatin-expressing (GIN) and fast-spiking inhibitory interneurons in UP-DOWN states of mouse neocortex. J Neurophysiol 104: 596-606, 2010. First published June 10, 2010; doi:10.1152/jn.00206.2010. The neocortex contains multiple types of inhibitory neurons whose properties suggest they may play different roles within the cortical circuit. By recording from three cell types during two distinct network states (UP and DOWN states) in vitro, we were able to quantify differences in firing characteristics between these cells during different network regimes. We recorded from regular-spiking (RS) excitatory cells and two types of inhibitory neurons, the fast-spiking (FS) neurons and GFP- (and somatostatin-) expressing inhibitory neurons (GIN), in layer 2/3 of slices from mouse somatosensory neocortex. Comparisons of firing characteristics between these cells during UP-and DOWN-states showed several patterns. First, of these cell types, only GIN cells fired persistently during DOWN-states, whereas all three cell types fired readily during UP-states. Second, the onset of firing and distribution of action potentials throughout UP-states differed by cell type, showing that FS cell UP-state firing occurred preferentially near the beginning of the UP-state, whereas the firing of RS cells was slower to develop at the start of the UP-state, and GIN cell firing was sustained throughout the duration of the UP-state. Finally, membrane potential and spike correlations between heterogeneous cell types were more pronounced during UP-states and, in the case of RS synapses onto GIN cells, varied throughout the UP-state. These results suggest that there is a division of labor between FS and GIN cells as the UP-state progresses and suggest that GIN cells could be important in the termination of UP-states.