GABA NEURONS IN THE SUPERFICIAL LAYERS OF THE RAT DORSAL COCHLEAR NUCLEUS - LIGHT AND ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY
GABA NEURONS IN THE SUPERFICIAL LAYERS OF THE RAT DORSAL COCHLEAR NUCLEUS - LIGHT AND ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY
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DOI：
10.1002/cne.902350106
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
MUGNAINI, E
中科院分区：
文献类型：
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作者：
MUGNAINI, E
用光镜和电子显微镜免疫细胞化学方法研究了大鼠耳蜗背核(DCN)浅层神经元的环路。针对固有标记谷氨酸脱羧酶(GAD)的抗血清被用来识别和定位使用GABA作为神经递质的轴突终末和神经元。DCN的第1层和第2层含有非常高密度的GABA能胞体，仅与耳蜗核腹侧核的颗粒细胞域相匹配，尤其是浅层颗粒细胞域。这两层还含有比耳蜗核复合体的所有其他大细胞区都高得多的GABA能细胞体。车轮和星状神经元，可能还有高尔基细胞，以前在高尔基和EM研究中的特征，似乎是免疫染色的，推测是抑制的。证实了平行纤维、颗粒细胞轴突与车轮神经元和星状神经元之间的突触关系。星状细胞通过缝隙连接相互连接。散布在第一层的是大的GABA能神经元，它们以不规则的频率出现，可能代表移位的浦肯野细胞，以前被识别为浦肯野细胞特异性标记物。颗粒神经元和锥体神经元没有染色，即使在局部注射秋水仙素后也是如此，这会增强其他GAD阳性细胞的免疫染色，因此必须使用不同于GABA的递质。根据高尔基体、EM和递质免疫细胞化学的资料，讨论了DCN的棘侧车轮和棘状星状细胞与小脑Purkinje和星状/篮状细胞之间的可能相似之处。
Light and electron microscopic immunocytochemistry were used to study the neuronal circuit of the superficial layers in the rat dorsal cochlear nucleus (DCN). An antiserum against the intrinsic marker glutamate decarboxylase (GAD) is used to identify and map axon terminals and neurons that use GABA as a neurotransmitter. Layers 1 and 2 of the DCN contain a very high density of GABAergic boutons, matched only by the granule cell domains of the ventral cochlear nucleus, especially the superficial granule cell domain. These 2 layers also contain much higher concentrations of GABAergic cell bodies than all other magnocellular regions of the cochlear nuclear complex. Cartwheel and stellate neurons, and probably also Golgi cells, previously characterized in Golgi and EM investigations, appear immunostained and are presumably inhibitory. The synaptic relations between parallel fibers, the axons of granule cells and cartwheel and stellate neurons are confirmed. Stellate cells are coupled to one another by gap junctions. Scattered in layer 1 are large, GABAergic neurons that occur with irregular frequency and presumably represent displaced Purkinje cells, previously identified with a Purkinje-cell-specific marker. Granule neurons and pyramidal neurons remain unstained, even after topical injection of colchicine, which enhances immunostaining of the other GAD-positive cells and therefore must use transmitters different from GABA. The possible analogies between the spiny cartwheel and the aspiny stellate cells of the DCN and the cerebellar Purkinje and stellate/basket cells are discussed in the light of data from Golgi, EM and transmitter immunocytochemistry.