RELATIONSHIP BETWEEN RNA SYNTHESIS AND LOOP STRUCTURE IN LAMPBRUSH CHROMOSOMES
RELATIONSHIP BETWEEN RNA SYNTHESIS AND LOOP STRUCTURE IN LAMPBRUSH CHROMOSOMES
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DOI：
10.1073/pnas.49.4.544
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发表时间：
1963-01-01
影响因子：
11.1
通讯作者：
MIRSKY, AE
中科院分区：
文献类型：
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作者：
IZAWA, M;ALLFREY, VG;MIRSKY, AE
核糖核酸在灯刷染色体中的合成发生在从染色体主轴突出的环上。除了RNA之外，这些环还含有DNA。根据对放线菌素D抑制的敏感性判断，染色体中RNA的合成是一个依赖DNA的过程。这种抗生素也会阻断核仁RNA的合成。将放线菌素D添加到分离的染色体或完整的卵母细胞中，不仅抑制RNA合成，而且导致染色体环的消失。修饰的放线菌素C3（不阻断RNA合成）不会引起环回缩。其他抑制核RNA合成的物质，如富含精氨酸的组蛋白，也会导致环消失。蛋白质合成的抑制剂，如嘌呤霉素，没有这种效果。结果表明，活性染色体位点的形态不仅与其合成RNA的能力密切相关，而且依赖于它。
Ribonucleic acid synthesis in lampbrush chromosomes occurs on loops which project from the main axis of the chromosome. These loops contain DNA in addition to RNA. The synthesis of RNA in the chromosome is a DNA-dependent process, as judged by its sensitivity to inhibition by Actino-mycin D. Nucleolar RNA synthesis is also blocked by this antibiotic. The addition of Actinomycin D to isolated chromosomes, or to intact oocytes, not only inhibits RNA synthesis, but also leads to a disappearance of the chromosomal loops. A modified Actinomycin C3 (which does not block RNA synthesis) does not cause loop retraction. Other agents which inhibit nuclear RNA synthesis, such as the arginine-rich histones, also cause the loops to disappear. Inhibitors of protein synthesis, such as puromycin, do not have this effect. The results suggest that the morphology of an active chromosomal site is not only closely related to its capacity to synthesize RNA, but is dependent upon it.