Prolonged valproic acid treatment does not reduce the size of latent HIV reservoir
Prolonged valproic acid treatment does not reduce the size of latent HIV reservoir
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DOI：
10.1097/qad.0b013e3282fd6ddc
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发表时间：
2008-06-19
期刊：
影响因子：
3.8
通讯作者：
Lambotte, Olivier
中科院分区：
文献类型：
--
作者：
Sagot-Lerolle, Nathalie;Lamine, Aurelia;Lambotte, Olivier
目的：为了探讨长期丙戊酸治疗对HIV水库的患者在高活性antiretroviral therapeutic.Design的影响：在一个单中心的试点研究，艾滋病毒水库的11例患者接受丙戊酸癫痫发作超过2年的大小进行了比较，与13个匹配的患者。此外，在法国的临床试验中接受丙戊酸治疗中断的患者的结果recorded.Methods：总和集成的HIV-1 DNA，分别，外周血单核细胞和CD 4 T细胞的患者进行了定量的实时PCR方法。通过定量有限稀释试验估计携带有复制能力病毒的CD 4 T细胞的频率，其中通过RT-PCR在活化的CD 4 T细胞的培养上清液中检测病毒生长。包括在预定的治疗中断试验接受丙戊酸的患者的临床图表review.Results：总的和集成的HIV DNA是convemically更丰富的细胞携带复制能力的病毒，但这三个参数之间没有显着差异两组匹配的患者。3例接受丙戊酸的患者被纳入计划的治疗中断试验。病毒复制的反弹是类似的其他患者的trials.Conclusion：长期丙戊酸治疗似乎是不够的，以减少HIV-1水库的大小。(c)2008年威科健康垂直酒吧利平科特威廉姆斯&威尔金斯。
Objective: To investigate the impact of prolonged valproic acid treatment on the HIV reservoir in patients on highly active antiretroviral therapy.Design: In a single-center pilot study, the size of the HIV reservoir of 11 patients receiving valproic acid for seizures for more than 2 years was compared with 13 matched patients. In addition, the outcome of patients receiving valproic acid in the French clinical trials of scheduled treatment interruption was recorded.Methods: Total and integrated HIV-1 DNA in, respectively, peripheral blood mononuclear cells and CD4 T cells of the patients were quantified by real-time PCR methods. The frequency of CD4 T cells carrying replication-competent virus was estimated by a quantitative limiting-dilution assay in which virus growth was detected by RT-PCR in culture supernatants of activated CD4 T cells. Clinical charts of the patients included in scheduled treatment interruption trials receiving valproic acid were reviewed.Results: Total and integrated HIV DNA were logarithmically more abundant than cells carrying replication-competent virus, but there was no significant difference in these three parameters between the two groups of matched patients. Three patients receiving valproic acid were included in scheduled treatment interruption trials. The rebound of viral replication was similar to that of the other patients of the trials.Conclusion: Long-term valproic acid therapy seems to be insufficient to reduce the size of the HIV-1 reservoir. (c) 2008 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.