Synthesis and circular dichroism of tetraarylporphyrin-oligonucleotide conjugates
Synthesis and circular dichroism of tetraarylporphyrin-oligonucleotide conjugates
复制标题
DOI：
10.1021/ja043373z
复制
发表时间：
2005-03-30
影响因子：
15
通讯作者：
Proni, G
中科院分区：
文献类型：
--
作者：
Balaz, M;Holmes, AE;Proni, G
本文报道了3'端含卟啉的自互补8聚体DNA序列的制备和圆二色性研究。连接在双链螺旋两端的两个卟啉之间的电子相互作用（发色团间距离在28 - 40 nm的范围内）在可见光区（400 - 450 nm）产生长距离激子耦合CD。卟啉发色团作为DNA骨架几何变化的灵敏探针，灵敏地反映了双链到单链的转变。这项研究表明，使用激子耦合卟啉CD的DNA构象研究的可能性。
The preparation and circular dichroic (CD) studies of self-complimentary 8-mer DNA sequences with a porphyrin at the 3‘ end are presented. Electronic interaction between the two porphyrins (the interchromophoric distance is in the range of 28−40 Å), attached to both ends of the double-stranded helix, gives rise to a long-range exciton-coupled CD in the visible region (400−450 nm). The porphyrin chromophores act as sensitive probes of geometrical changes in the DNA backbone and sensitively reflect the double-strand to single-strand transition. This study demonstrates the possibility of using exciton-coupled porphyrin CDs for conformational studies of DNA.