Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata
Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata
复制标题
海参四种硫酸化多糖的纯化、结构表征及抗凝血活性
DOI：
10.1016/j.ijbiomac.2020.08.150
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发表时间：
2020-12-01
影响因子：
8.2
通讯作者：
Zhao, Jinhua
中科院分区：
文献类型：
--
作者：
Gao, Na;Chen, Ru;Zhao, Jinhua
海参硫酸多糖具有独特的化学结构和多种生物活性。采用阴离子交换层析和化学解聚法从黑乳海参体壁中分离纯化了4种硫酸多糖。经理化和结构分析鉴定为硫酸岩藻聚糖（SFI，SFII）、岩藻糖基化硫酸软骨素（FCS）和硫酸氨基聚糖（AG）。SFI、SFII、FCS和AG的分子量分别为470.6、36.8、42.6和39.6 kDa，硫酸根含量分别为21.40%、35.86%、33.70%和35.70%。通过单糖组成和1D/2D NMR谱分析，确定了它们的一级结构。结果，FCS和SFII的重复序列分别为-> 4）-[L-Fuc（3S 4S）-（α 1 -> 3）]-D-GlcA-（β 1 -> 3）-D-GalNAc（4S 6S）-（β 1 ->和-> 4-L-Fuc-（3SO（3）-）-α 1 ->。SFI的一级结构为-> 3）-L-Fuc（2S 4S）-（α 1 -> 4）-L-Fuc-（α 1 -> 3）-L-Fuc（2S）-（α 1 -> 4）-L-Fuc-（α 1-> 4）。硫酸化AG由四种单糖组成。体外抗凝血活性进一步评价。FCS和AG具有较强的抗凝活性和内因子Xase抑制活性。这些结果扩展了对海参硫酸多糖结构类型的认识，并进一步阐明了其功能性。(C)2020爱思唯尔B. V.保留所有权利。
Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities. In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization. They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses. The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively. Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis. As a result, the repeating sequences of FCS and SFII were -> 4)-[L-Fuc(3S4S)-(alpha 1 -> 3)]-D-GlcA-(beta 1 -> 3)-D-GalNAc(4S6S)-(beta 1 -> and -> 4-L-Fuc-(3SO(3)-)-alpha 1 ->, respectively. The primary structure of SFI was -> 3)-L-Fuc(2S4S)-(alpha 1 -> 4)-L-Fuc-(alpha 1 -> 3)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc-(alpha 1 ->. The sulfated AG was composed of four types of monosaccharides. Their anticoagulant activities were further evaluated in vitro. FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity. These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality. (C) 2020 Elsevier B.V. All rights reserved.