A PNA–DNA hybridization chip approach for the detection of β-secretase activity
A PNA–DNA hybridization chip approach for the detection of β-secretase activity
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DOI：
10.1016/j.bmcl.2005.10.064
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发表时间：
2006-02
影响因子：
2.7
通讯作者：
Shusuke Sano;K. Tomizaki;K. Usui;H. Mihara
中科院分区：
文献类型：
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作者：
Shusuke Sano;K. Tomizaki;K. Usui;H. Mihara
开发的可寻址芯片技术基于 PNA-DNA 互补杂交，配备短的七聚体 PNA 编码肽，可以成为监测芯片上免疫测定的多功能支架。我们还开发并验证了一种以高灵敏度方式进行 β-分泌酶测定的方法，结果是，束缚双荧光探针的肽底物使我们能够检测 β-分泌酶活性，其灵敏度比溶液中的测定高 10 倍。
Developed was the addressable chip technology based on the PNA–DNA complementary hybridization equipped with short seven-mer PNA-encoded peptides that can be a versatile scaffold to monitor on-chip immunoassays. We also developed and validated a methodology to perform β-secretase enzyme assay with a highly sensitive fashion, resulting that a peptide substrate tethering dual fluorescent probes allowed us to detect β-secretase activity 10 times more sensitively than assays in solution.