Feasibility of the Optical Imaging of Thrombus Formation in a Rotary Blood Pump by Near-Infrared Light
Feasibility of the Optical Imaging of Thrombus Formation in a Rotary Blood Pump by Near-Infrared Light
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DOI：
10.1111/aor.12377
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发表时间：
2014-09-01
影响因子：
2.4
通讯作者：
Maruyama, Osamu
中科院分区：
文献类型：
--
作者：
Sakota, Daisuke;Murashige, Tomotaka;Maruyama, Osamu
血液凝固是使用机械循环支持设备（如血泵）时的主要问题之一。血栓形成的非侵入性检测和成像不仅对开发更多的血液相容性设备有用，而且对血液凝固的管理以避免梗死的风险也有用。本研究的目的是调查使用近红外光成像的血栓形成的旋转血泵。使用高光谱成像（HSI）系统分析了血栓在600至750 nm波长范围内的光学特性。使用了专门设计的具有可见底部区域的流体动力悬浮离心血泵。使用牛全血对泵进行了5次体外抗血栓形成试验，其中在实验前将活化凝血时间调整为200 s。两个卤素灯用于光源。使用HSI系统对通过泵的前向散射和泵底部区域上的后向散射成像。HSI显示在血栓形成位置在670至750 nm波长范围内的前向散射增加。可以检测到以2780转/分旋转的叶轮中血栓开始形成的时间。利用全血和血栓之间的光谱差异对血栓形成进行成像。结果表明，在旋转血泵中动态检测和成像血栓形成的可行性。
Blood coagulation is one of the primary concerns when using mechanical circulatory support devices such as blood pumps. Noninvasive detection and imaging of thrombus formation is useful not only for the development of more hemocompatible devices but also for the management of blood coagulation to avoid risk of infarction. The objective of this study is to investigate the use of near-infrared light for imaging of thrombus formation in a rotary blood pump. The optical properties of a thrombus at wavelengths ranging from 600 to 750nm were analyzed using a hyperspectral imaging (HSI) system. A specially designed hydrodynamically levitated centrifugal blood pump with a visible bottom area was used. In vitro antithrombogenic testing was conducted five times with the pump using bovine whole blood in which the activated blood clotting time was adjusted to 200s prior to the experiment. Two halogen lights were used for the light sources. The forward scattering through the pump and backward scattering on the pump bottom area were imaged using the HSI system. HSI showed an increase in forward scattering at wavelengths ranging from 670 to 750nm in the location of thrombus formation. The time at which the thrombus began to form in the impeller rotating at 2780rpm could be detected. The spectral difference between the whole blood and the thrombus was utilized to image thrombus formation. The results indicate the feasibility of dynamically detecting and imaging thrombus formation in a rotary blood pump.