ORGANIZATION OF THE GENE FOR HUMAN ERYTHROCYTE-MEMBRANE PROTEIN-4.2 - STRUCTURAL SIMILARITIES WITH THE GENE FOR THE A SUBUNIT OF FACTOR-XIII
ORGANIZATION OF THE GENE FOR HUMAN ERYTHROCYTE-MEMBRANE PROTEIN-4.2 - STRUCTURAL SIMILARITIES WITH THE GENE FOR THE A SUBUNIT OF FACTOR-XIII
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DOI：
10.1073/pnas.88.11.4840
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发表时间：
1991-06-01
影响因子：
11.1
通讯作者：
COHEN, CM
中科院分区：
文献类型：
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作者：
KORSGREN, C;COHEN, CM
人红细胞带4.2是一种主要的膜相关蛋白，在红细胞存活中具有重要但尚未确定的作用。 我们先前对条带4.2的完整cDNA进行了测序，结果表明该蛋白与转氨酶家族蛋白具有很强的序列同一性，但缺乏转氨酶活性。 在这里，我们分析了条带4.2的基因组组织。 条带4.2基因由13个外显子和12个内含子组成，几乎等于20个酶。 网织红细胞包含两种不同大小的条带4.2的信息，我们的研究结果表明，主要的，较小的，信息是由条带4.2外显子I内的选择性剪接产生的。 该基因的上游区域具有几个预期的启动子元件，其排列模式类似于另外两个红细胞基因β-珠蛋白和胆色素原脱氨酶。 条带4.2氨基酸序列与人凝血因子XIII的a亚基的氨基酸序列的比对以及序列到外显子的划分揭示了配对外显子的大小的显著对应性，并且在大多数情况下是同一性。 此外，两个基因的每个相应内含子属于相同的剪接点类别。这些和其他相似性表明，带4.2的基因与因子XIII的a亚基密切相关，并可能来源于因子XIII的a亚基，这些蛋白质可能具有共同的结构和功能特性。
Human erythrocyte band 4.2 is a major membrane-associated protein with an important, but still undefined, role in erythrocyte survival. We previously sequenced the complete cDNA for band 4.2 and showed that the protein has a strong sequence identity with the transglutaminase family of proteins but lacks transglutaminase activity. Here we have analyzed the genomic organization of band 4.2. The band 4.2 gene is almost-equal-to 20 kilobases, consisting of 13 exons and 12 introns. Reticulocytes contain two different sized messages for band 4.2, and our results show that the major, smaller, message is produced by alternative splicing within band 4.2 exon I. The upstream region of the gene has several prospective promoter elements arranged in a pattern similar to that of two other erythroid genes, beta-globin and porphobilinogen deaminase. Alignment of the band 4.2 amino acid sequence with that of the a subunit of human coagulation factor XIII and division of the sequences into exons reveal a remarkable correspondence, and in most cases identity, in the sizes of the paired exons. Moreover, each corresponding intron of the two genes is of an identical splice junction class. These and other similarities suggest that the gene for band 4.2 is closely related to and possibly derived from that for the a subunit of factor XIII and that the proteins may share common structural and functional properties.