Transcriptomic analyses during development reveal mechanisms of integument structuring and color production
Transcriptomic analyses during development reveal mechanisms of integument structuring and color production
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DOI：
10.1007/s10682-023-10256-2
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发表时间：
2023-09-22
影响因子：
1.9
通讯作者：
Macmanes，Matthew D.
中科院分区：
文献类型：
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作者：
Stuckert，Adam M. M.;Freeborn，Layla;Macmanes，Matthew D.
皮肤的颜色和图案在动物的生存和繁殖中起着关键作用。因此，颜色表型产生了强烈的研究兴趣。在警戒物种中，颜色表型对于避免捕食和择偶很重要。然而，我们仍然对颜色产生的潜在遗传机制知之甚少，特别是在一些模式生物之外。在这里，我们试图了解不同颜色产生的遗传机制，以及这些在整个发育过程中如何经历变化的表达模式。为了回答这个问题，我们研究了两种不同颜色斑块的基因表达，我们从毒蛙Oophaga pumilio幼蝌蚪到成年蝌蚪的发育时间序列中，发现了6个在每个发育阶段的色块之间差异表达的基因，（casq 1，hand 2，myh 8，prva，tbx 3，和zic 1）。其中，hand 2，myh 8，tbx 3，Casq 1和prvaburic 1分别是Ca 2+转运蛋白和Ca 2+转运蛋白，并且可能在防止对抑制Ca 2 +-ATP酶活性的短小毒素的自毒性中起作用。我们鉴定了进一步的候选基因（例如，ADH、ALDH 1A 2、ASIP、LEF 1、MC 1 R、Tyr、TyrP 1、XDH），并鉴定一组可能在发育期间的外皮重组中起关键作用的中枢基因（例如，I-IV型胶原蛋白基因、赖氨酰氧化酶），其也可以通过有助于颜色和图案的色素团的结构组织来影响着色。总体而言，我们确定了一套候选基因在生产不同的颜色类型的多型，警戒物种的推定作用。
Skin coloration and patterning play a key role in animal survival and reproduction. As a result, color phenotypes have generated intense research interest. In aposematic species, color phenotypes can be important in avoiding predation and in mate choice. However, we still know little about the underlying genetic mechanisms of color production, particularly outside of a few model organisms. Here we seek to understand the genetic mechanisms underlying the production of different colors and how these undergo shifting expression patterns throughout development. To answer this, we examine gene expression of two different color patches(yellow and green) in a developmental time series from young tadpoles through adults in the poison frogOophaga pumilio.We identified six genes that were differentially expressed between color patches in every developmental stage (casq1, hand2, myh8, prva, tbx3,andzic1).Of these,hand2, myh8, tbx3,andzic1have either been identified or implicated as important in coloration in other taxa.Casq1andprvabuffer Ca2+and are a Ca2+transporter, respectively, and may play a role in preventing autotoxicity to pumiliotoxins, which inhibit Ca2+-ATPase activity. We identify further candidate genes (e.g.,adh, aldh1a2, asip, lef1, mc1r, tyr, tyrp1, xdh), and identify a suite of hub genes that likely play a key role in integumental reorganization during development (e.g., collagen type I–IV genes, lysyl oxidases) which may also affect coloration via structural organization of chromatophores that contribute to color and pattern. Overall, we identify the putative role of a suite of candidate genes in the production of different color types in a polytypic, aposematic species.