Intracerebroventricular injection of L-arginine induces sedative and hypnotic effects under an acute stress in neonatal chicks
Intracerebroventricular injection of L-arginine induces sedative and hypnotic effects under an acute stress in neonatal chicks
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DOI：
10.1007/s00726-007-0610-4
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发表时间：
2008-06-01
期刊：
影响因子：
3.5
通讯作者：
Furuse, M.
中科院分区：
文献类型：
--
作者：
Suenaga, R.;Tomonaga, S.;Furuse, M.
L-精氨酸参与许多与生物体正常生理相关的重要且多样化的生化反应。在本研究中，我们研究了L-精氨酸中枢给药对新生雏鸡应激反应的影响及其机制。脑室内 (i.c.v.) 注射 L-精氨酸明显以剂量依赖性方式减弱应激反应，并在 10 分钟内诱导睡眠样行为。为了阐明 L-精氨酸对雏鸡产生镇静和催眠作用的机制，我们研究了一氧化氮 (NO) 合酶 (NOS) 抑制剂对 L-精氨酸诱导的镇静和催眠作用的影响，以及 NO 供体的影响。 L-精氨酸（1.9 μ mol）诱导的镇静和催眠作用通过静脉注射减弱。与非选择性 NOS 抑制剂 N-G-硝基-L-精氨酸甲酯 HCl (400 nmol) 共注射。此外，NOS 抑制剂 N-G-硝基-d-精氨酸甲酯盐酸盐 (400 nmol) 的无活性异构体略微削弱了 L-精氨酸的作用。 i.c.v.注射3-吗啉代诺胺盐酸盐（一种自发的NO供体）对姿势影响不大。 i.c.v.注射L-精氨酸对不同脑部位的NOx浓度没有影响。这些结果表明，通过 NOS 生成 NO 在 L-精氨酸的镇静和催眠作用中的贡献可能较低。因此，L-精氨酸和/或其代谢物（不包括NO）可能是这些作用所必需的。
L-Arginine participates in many important and diverse biochemical reactions associated with the normal physiology of the organism. In the present study, we investigated the effect of central administration of L-arginine on the stress response and its mechanism in neonatal chicks. Intracerebroventricular (i.c.v.) injection of L-arginine clearly attenuated the stress response in a dose-dependent manner, and induced sleep-like behavior during 10 min. To clarify the mechanism by which L-arginine induces sedative and hypnotic effects in chicks, we investigated the effects of nitric oxide (NO) synthase (NOS) inhibitors on L-arginine-induced sedative and hypnotic effects, and as well as the effects of a NO donor. L-Arginine-induced (1.9 mu mol) sedative and hypnotic effects were attenuated by i.c.v. co-injection with a non-selective NOS inhibitor N-G-nitro-L-arginine methyl ester HCl (400 nmol). In addition, the effects of L-arginine were slightly attenuated by the inactive isomer of the NOS inhibitor N-G-nitro-d-arginine methyl ester HCl (400 nmol). The i.c.v. injection of 3-morpholinosylnomine hydrochloride, a spontaneous NO donor, had little effect on postures. The i.c.v. injection of L-arginine had no effect on NOx concentration at various brain sites. These results suggested that the contribution of NO generation via NOS may be low in the sedative and hypnotic actions of L-arginine. Therefore, L-arginine and/or its metabolites, excluding NO, may be necessary for these actions.