In vitro inhibition of postharvest pathogens of fruit and control of gray mold of strawberry and green mold of citrus by aureobasidin A
In vitro inhibition of postharvest pathogens of fruit and control of gray mold of strawberry and green mold of citrus by aureobasidin A
复制标题
金黄木素A体外抑制水果采后病原菌及防治草莓灰霉病和柑橘绿霉病
DOI：
10.1016/j.ijfoodmicro.2007.07.054
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发表时间：
2007-11-01
影响因子：
5.4
通讯作者：
Li, Hongye
中科院分区：
文献类型：
--
作者：
Liu, Xiaoping;Wang, Jiye;Li, Hongye
ABA(Aureobasidin A，ABA)是由出芽短梗霉R106产生的一种抗真菌环肽抗生素，已被证明对多种真菌和原生动物有效。本文报道了ABA对引起多种水果采后病害的五种病原真菌：指状青霉、意大利青霉、膨胀青霉、灰霉病菌和果实假单胞菌的孢子萌发、芽薯伸长和菌丝生长的抑制作用。ABA通过降低分生孢子萌发率、延缓分生孢子萌发起始、抑制芽管和菌丝的伸长以及诱导真菌生殖管和菌丝形态的异常变化来抑制5种病原真菌。这些真菌对ABA的敏感性因病原菌种类而异。洋地老虎最敏感，果蝇最不敏感。结果表明，50µg/ml的ABA对人工接种柑橘青霉病和草莓灰霉病的发病率和严重程度均有一定的抑制作用。因此，ABA和/或其类似物有望成为控制水果采后腐烂的相对安全和有前景的杀菌剂候选者，因为ABA的靶标是肌醇磷酸神经酰胺合成酶(IPC)，这是真菌所必需但哺乳动物不存在的一种酶。(C)2007 Elsevier B.V.保留所有权利。
Aureobasidin A (AbA), an antifungal cyclic depsipeptide antibiotic produced by Aureobasidium pullulans R 106, has previously been shown to be effective against a wide range of fungi and protozoa. Here we report the inhibitory effects of AbA on spore germination, germ tuber elongation and hyphal growth of five pathogenic fungi including Penicillium digitatum, P. italicum, P. expansum, Botrytis cinerea and Monilinia fructicola, which are major pathogens causing postharvest diseases of a variety of fruits. AbA inhibited five pathogenic fungi by reducing conidial germination rates, delaying conidial germination initiation, restricting elongation of germ tuber and mycelium, as well as inducing abnormal alternations of morphology of genii tubes and hyphae of these fungi. The sensitivity of these fungi to AbA was pathogen species-dependent. P digitatum was the most sensitive and M.fructicola the least. Importantly, AbA at 50 mu g/ml was effective in controlling the citrus green mold and in reducing the strawberry gray mold incidence and severity, caused by P. digitatum and B. cinerea, respectively, after artificial inoculation. AbA and/or its analogs, therefore, hold promise as relatively safe and promising fungicide candidates to control postharvest decays of fruits, because AbA targets the inositol phosphorylceramide (IPC) synthase, an enzyme essential for fungi but absent from mammals. (C) 2007 Elsevier B.V. All rights reserved.