Immortalization of primary rat cells by human papillomavirus type 16 subgenomic DNA fragments controlled by the SV40 promoter.
Immortalization of primary rat cells by human papillomavirus type 16 subgenomic DNA fragments controlled by the SV40 promoter.
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由 SV40 启动子控制的人乳头瘤病毒 16 型亚基因组 DNA 片段使原代大鼠细胞永生化。
DOI：
10.1016/0042-6822(88)90694-0
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
K. Yoshiike
中科院分区：
文献类型：
--
作者：
T. Kanda;S. Watanabe;K. Yoshiike
我们使用含有新霉素耐药诱导单位(PSV2neo)和由SV40早期启动子控制的HPV16亚基因组DNA片段的转录单位的表达载体，检测了HPV16早期基因使原代大鼠脑细胞永生化或转化的能力。转染后，通过再喂食和重复鉴定的方式维持耐药克隆。单独的E7基因被发现能够使原代大鼠细胞永生化和形态转化，转化的细胞表现出锚定无关的生长。虽然它的活性低于E7基因，但E6基因也使原代大鼠细胞永生化。永生化或转化的细胞含有HPV16特异性DNA和mRNA。
We tested the human papillomavirus 16 (HPV 16) early genes for their ability to immortalize or transform primary rat brain cells, using the expression plasmids that contain the neomycin-resistance-inducing unit (pSV2neo) and the transcriptional unit for the HPV 16 subgenomic DNA fragments controlled by the SV40 early promoter. After transfection, drug-resistant colonies were maintained by refeeding and replating for characterization. The E7 gene alone was found to be capable of immortalizing and morphologically transforming primary rat cells, and the transformed cells showed anchorage-independent growth. Although its activity was lower than that of the E7 gene, the E6 gene also immortalized primary rat cells. The immortalized or transformed cells contained HPV 16-specific DNA and mRNA.