Transcriptomic analysis of Rongchang pig brains and livers
Transcriptomic analysis of Rongchang pig brains and livers
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荣昌猪脑和肝脏的转录组分析
DOI：
10.1016/j.gene.2015.01.051
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发表时间：
2015-04-10
期刊：
影响因子：
3.5
通讯作者：
He, Ningjia
中科院分区：
文献类型：
--
作者：
Gan, Ling;Xie, Liwei;He, Ningjia
高通量RNA测序（RNA-seq）技术的最新发展对我们理解哺乳动物转录组的结构和表达谱产生了巨大影响。为了深入了解猪生产和生物医学模型的有用性，使用RNA-seq技术对荣昌猪脑和肝脏的转录组谱进行了表征，以发现功能候选分子。本研究对荣昌猪脑和肝脏的总RNA进行了测序，获得了8.6Gb的测序数据。该分析通过分别鉴定脑和肝中的5575和4600个差异表达基因（DEG）以及DEG的功能分析揭示了组织特异性。此外，还鉴定了83个神经肽基因转录本、69个神经肽受体基因转录本、10个前神经肽转化酶基因转录本和许多其他神经肽相关蛋白基因转录本。荣昌猪脑和肝脏的转录谱基本具备特征。(C)2015 Elsevier B. V.版权所有。
Recent developments in high-throughput RNA sequencing (RNA-seq) technology have led to a dramatic impact on our understanding of the structure and expression profiles of the mammalian transcriptome. To gain insights into the usefulness of swine production and biomedical model, the transcriptome profiling of Rongchang pig brains and livers was characterized using RNA-seq technology to uncover functional candidate molecules. In the study, total RNAs from brains and livers of Rongchang pig were sequenced and 8.6 Gb sequencing data was obtained. This analysis revealed tissue specificity through the identification of 5575 and 4600 differentially expressed genes (DEGs) in brains and livers, respectively and the functional analysis of DEGs. Furthermore, 83 neuropeptide gene transcripts, 69 neuropeptide receptor gene transcripts, 10 pro-neuropeptide convertase gene transcripts and many other neuropeptide related protein gene transcripts were identified. Totally, the major characteristics of the transcriptional profiles of Rongchang pig brains and livers were present. (C) 2015 Elsevier B.V. All rights reserved.