IMMOBILIZED LECTIN COLUMNS - USEFUL TOOLS FOR THE FRACTIONATION AND STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDES
IMMOBILIZED LECTIN COLUMNS - USEFUL TOOLS FOR THE FRACTIONATION AND STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDES
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DOI：
10.1016/0021-9673(92)80101-y
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发表时间：
1992-04-24
期刊：
影响因子：
--
通讯作者：
ENDO, T
中科院分区：
文献类型：
--
作者：
KOBATA, A;ENDO, T
阐明伴刀豆球蛋白 A Sepharose 柱的结合特异性使得使用固定凝集素柱对寡糖和糖肽进行亲和层析成为可能。随后将固定化红细胞凝集植物血凝素、Aleuria aurantia 凝集素、曼陀罗凝集素、蓖麻凝集素和 Allomyrina dichotoma 凝集素添加到一系列经过充分表征的凝集素柱中，提供了一种分离 N-连接寡糖混合物的方法，甚至 单个组件。
Elucidation of the binding specificity of a concanavalin A Sepharose column led to the possibility of the affinity chromatography of oligosaccharides and glycopeptides with the use of immobilized lectin columns. Subsequent addition of immobilized erythroagglutinating phytohaemagglutinin, Aleuria aurantia lectin, Datura stramonium agglutinin, Ricinus communis agglutinin and Allomyrina dichotoma agglutinin to the range of well characterized lectin columns has afforded a way to fractionate a mixture of N-linked oligosaccharides even to a single component.