cAMP-dependent protein kinase (PKA) subunit mRNA levels in postmortem brain from patients with bipolar affective disorder (BD)
cAMP-dependent protein kinase (PKA) subunit mRNA levels in postmortem brain from patients with bipolar affective disorder (BD)
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DOI：
10.1016/s0169-328x(03)00211-0
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发表时间：
2003-08-19
期刊：
影响因子：
--
通讯作者：
Warsh, JJ
中科院分区：
文献类型：
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作者：
Chang, A;Li, PP;Warsh, JJ
早期的研究结果升高的基础和刺激PKA的活动，并增加免疫反应性水平的PKA调节和催化亚基在离散死后的双相情感障碍（BD）患者的脑区表明，PKA的干扰参与BD的病理生理。使用SYBR绿色实时RT-PCR测量PKA亚基mRNA水平，以确定先前观察到的PKA RII β和Calpha亚基免疫反应性水平的差异是否与来自相同BD患者和匹配对照的颞叶和额叶皮质中mRNA水平的相应变化相关。与先前报道的BD脑中PKA亚基的较高免疫标记水平形成鲜明对比，BD患者的颞叶和额叶皮质中RIIbeta和Calpha亚基mRNA水平与对照组相比无显著差异。这些研究结果推断，升高PKA免疫标记和活性发现在选定的大脑皮层区域的BD死后的大脑是由于转录后机制，而不是在调节基因转录和/或mRNA的PKA亚基的稳定性的变化。(C)2003 Elsevier B. V.保留所有权利。
Earlier findings of elevated basal and stimulated PKA activities, and increased immunoreactive levels of PKA regulatory and catalytic subunits in discrete postmortem brain regions from bipolar disorder (BD) patients suggest that disturbances in PKA are involved in the pathophysiology of BD. PKA subunit mRNA levels were measured using SYBR Green real-time RT-PCR to determine if previously observed differences in immunoreactive levels of PKA RIIbeta and Calpha subunits were associated with corresponding changes in mRNA levels in temporal and frontal cortices from the same BD patients and matched controls. In distinct contrast to the higher immunolabeling levels of the PKA subunits previously reported in the BD brain, there were no significant differences in RIIbeta and Calpha subunit mRNA levels in the temporal and frontal cortices of BD patients compared with controls. These findings infer that the elevated PKA immunolabeling and activity found in the selected cerebral cortical regions of BD postmortem brain were due to a posttranscriptional mechanism, rather than changes in regulation of gene transcription and/or mRNA stability of the PKA subunits. (C) 2003 Elsevier B.V. All rights reserved.