A role for alcohol dehydrogenase in the Drosophila immune response?
A role for alcohol dehydrogenase in the Drosophila immune response?
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DOI：
10.1111/j.1365-2583.2004.00543.x
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发表时间：
2005-04
影响因子：
2.6
通讯作者：
T. Schlenke;K. McKean
中科院分区：
文献类型：
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作者：
T. Schlenke;K. McKean
在最近的一项研究中，果蝇幼虫被注射了悬浮在1%乙醇中的细菌脂多糖（LPS），并鉴定了差异诱导的蛋白质组分。几种蛋白质的水平，包括乙醇脱氢酶（ADH），在LPS处理的苍蝇中增加，并被标记为免疫反应蛋白。然而，因为对照幼虫没有单独注射乙醇，所以鉴定的蛋白质可以代表对乙醇的响应。在这里，我们给果蝇幼虫注射了乙醇和LPS的混合物。虽然接受1%乙醇的幼虫中ADH活性增加，但注射LPS后其活性并未增加。这些结果表明，ADH在果蝇的免疫反应中不起作用，而在以前的研究中发现的其他蛋白质可能反而介导了果蝇和其他生物体的乙醇耐受性。
In a recent study Drosophila larvae were injected with bacterial lipopolysaccharide (LPS) suspended in 1% ethanol and differentially induced protein fractions were identified. The levels of several proteins, including alcohol dehydrogenase (ADH), increased in LPS‐treated flies and were labelled as immune response proteins. However, because control larvae were not injected with ethanol alone the identified proteins could represent a response to ethanol. Here, we injected Drosophila larvae with combinations of ethanol and LPS. While ADH activity increased in larvae receiving 1% ethanol, it was not increased after LPS injection. These results suggest that ADH plays no role in the Drosophila immune response, and that other proteins identified in the previous study may instead mediate ethanol tolerance in flies and other organisms.