An on-line archive of reconstructed hippocampal neurons
An on-line archive of reconstructed hippocampal neurons
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DOI：
10.1016/s0165-0270(98)00091-0
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发表时间：
1998-10-01
影响因子：
3
通讯作者：
Wheal, HV
中科院分区：
文献类型：
--
作者：
Cannon, RC;Turner, DA;Wheal, HV
我们已经开发了一个在线档案的神经元几何形状，鼓励使用逼真的树突状结构的形态和神经元建模，位于网址www.neuro.soton.ac.uk。最初，我们已经包括了完整的三维表示的87个神经元从海马，获得细胞内染色与biocytin和重建使用Neurolucida。档案系统包括一个结构编辑器，用于纠正与有效分支几何形状的任何偏差，并允许纠正数字化中的简单错误。该编辑器采用了一种独立于平台的文件格式，该格式强制执行了不应该有孤立分支和闭合循环的约束。它还集成了存档格式和各种神经元重建和建模软件包所使用的格式之间的相互转换软件。来自数字化软件的原始数据可以包括在存档中，以及编辑的重建和任何可用的进一步信息。交叉引用的表格和索引会自动更新，并根据一些字段进行排序，包括单元格类型、贡献者、提交日期和发布的参考文献。档案和结构编辑器都应该促进海马和其他大脑区域神经元的全三维重建的定量使用。(C)1998 Elsevier Science B.V.保留所有权利。
We have developed an on-line archive of neuronal geometry to encourage the use of realistic dendritic structures in morphometry and for neuronal modeling, located at web address www.neuro.soton.ac.uk. Initially we have included full three-dimensional representations of 87 neurons from the hippocampus, obtained following intracellular staining with biocytin and reconstruction using Neurolucida. The archive system includes a structure editor for correcting any departures from valid branching geometry and which allows simple errors in the digitisation to be corrected. The editor employs a platform-independent file format which enforces the constraints that there should be no isolated branches and no closed loops. It also incorporates software for interconversion between the archive format and those used by various neuronal reconstruction and modelling packages. The raw data from digitisation software can be included in the archive as well as edited reconstructions and any further information available. Cross-referenced tables and indexes are updated automatically and are sorted according to a number of fields including the cell type, contributor, submission date and published reference. Both the archive and the structure editor should facilitate the quantitative use of full three-dimensional reconstructions of neurons from the hippocampus and other brain regions. (C) 1998 Elsevier Science B.V. All rights reserved.