In Vivo Tomographic Imaging of Near-Infrared Fluorescent Probes
In Vivo Tomographic Imaging of Near-Infrared Fluorescent Probes
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DOI：
10.1162/153535002320162732
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发表时间：
2002-04-01
影响因子：
2.8
通讯作者：
Weissleder, Ralph
中科院分区：
文献类型：
--
作者：
Ntziachristos, Vasilis;Bremer, Christoph;Weissleder, Ralph
荧光成像越来越多地被用来探测活体动物的蛋白质功能和基因表达。这项技术可以加强对发病机制、药物开发和治疗干预的研究。在本文中，我们主要关注利用荧光介导的分子断层成像(FMT)进行三维荧光观察，这是一种新的成像技术，可以通过重建荧光探针在体内的分布来解析深层组织中的分子功能。我们将FMT的结果与传统的荧光反射成像(FRI)进行了比较，以研究裸鼠皮下移植瘤的蛋白酶功能。FMT和FRI的验证证明了这两种技术所确定的荧光色素激活的空间一致性。
Fluorescence imaging is increasingly used to probe protein function and gene expression in live animals. This technology could enhance the study of pathogenesis, drug development, and therapeutic intervention. In this article, we focus on three-dimensional fluorescence observations using fluorescence-mediated molecular tomography (FMT), a novel imaging technique that can resolve molecular function in deep tissues by reconstructing fluorescent probe distributions in vivo. We have compared FMT findings with conventional fluorescence reflectance imaging (FRI) to study protease function in nude mice with subsurface implanted tumors. This validation of FMT with FRI demonstrated the spatial congruence of fluorochrome activation as determined by the two techniques.