The DnaK chaperone system facilitates 30S ribosomal subunit assembly
The DnaK chaperone system facilitates 30S ribosomal subunit assembly
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DOI：
10.1016/s1097-2765(02)00562-2
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发表时间：
2002-07-01
期刊：
影响因子：
16
通讯作者：
Culver, GM
中科院分区：
文献类型：
--
作者：
Maki, JA;Schnobrich, DJ;Culver, GM
功能性大肠杆菌 30S 核糖体亚基可以在体外重建。然而，缓慢的动力学和尖锐的温度依赖性表明体内存在额外的组装因子。体外组装的提取物激活导致 DnaK/hsp70 分子伴侣组分与前 30S 颗粒结合。纯化的 DnaK、其辅助伴侣 DnaJ 和 GrpE 以及 ATP 可以在其他不允许的条件下促进功能性 30S 亚基的重建。在体内和体外观察到 DnaK、30S 亚基成分和核糖体生物发生之间存在联系。这些研究揭示了 DnaK/hsp70 伴侣系统除了在蛋白质折叠中的作用外，还具有新的作用，并表明可以促进 30S 亚基组装。
Functional Escherichia coli 30S ribosomal subunits can be reconstituted in vitro. However, slow kinetics and sharp temperature dependence suggest additional assembly factors are present in vivo. Extract activation of in vitro assembly results in association of DnaK/hsp70 chaperone components with pre-30S particles. Purified DnaK, its cochaperones DnaJ and GrpE, and ATP can facilitate reconstitution of functional 30S subunits under otherwise nonpermissive conditions. A link has been observed between DnaK, 30S subunit components, and ribosome biogenesis in vivo as well as in vitro. These studies reveal a novel role for the DnaK/hsp70 chaperone system, in addition to its well-documented role in protein folding, and suggest that 30S subunit assembly can be facilitated.