Skin regeneration with all accessory organs following ablation with irreversible electroporation
Skin regeneration with all accessory organs following ablation with irreversible electroporation
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DOI：
10.1002/term.2374
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发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Yarmush, Martin L.
中科院分区：
文献类型：
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作者：
Golberg, Alexander;Villiger, Martin;Yarmush, Martin L.
皮肤瘢痕形成是一个复杂的过程，导致形成致密的细胞外基质（ECM），而没有正常的皮肤附属物，如头发和腺体。在成年哺乳动物中缺乏无瘢痕愈合模型阻碍了成功疗法的发展。我们发现，不可逆的电穿孔皮肤驱动其再生与所有附属器官在正常成年大鼠。500 V的脉冲电场，70 μ s的脉冲持续时间和1000个3Hz的脉冲，通过两个相隔2 mm的电极施加，导致大量细胞死亡。然而，皮肤的ECM结构被保留。术后6个月，整个切除区的表皮、皮脂腺、肉脂膜、毛囊、微血管和皮利均得到重建。这些结果表明，在无瘢痕伤口愈合过程中，ECM结构在细胞的分化、迁移和信号传导中起关键作用。版权所有（C）2016约翰威利父子有限公司
Skin scar formation is a complex process that results in the formation of dense extracellular matrix (ECM) without normal skin appendages such as hair and glands. The absence of a scarless healing model in adult mammals prevents the development of successful therapies. We show that irreversible electroporation of skin drives its regeneration with all accessory organs in normal adult rats. Pulsed electric fields at 500V, with 70 mu s pulse duration and 1000 pulses delivered at 3Hz, applied through two electrodes separated by 2 mm lead to massive cell death. However, the ECM architecture of the skin was preserved. Six months after the ablation, the epidermis, sebaceous glands, panniculus carnosus, hair follicles, microvasculature and arrector pili muscle were altogether re-formed in the entire ablated area. These results suggest a key role of the ECM architecture in the differentiation, migration and signalling of cells during scarless wound healing. Copyright (C) 2016 John Wiley & Sons, Ltd.