Adenosine and behavioral state control: adenosine increases c-Fos protein and AP1 binding in basal forebrain of rats
Adenosine and behavioral state control: adenosine increases c-Fos protein and AP1 binding in basal forebrain of rats
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DOI：
10.1016/s0169-328x(99)00219-3
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发表时间：
1999-11-10
期刊：
影响因子：
--
通讯作者：
McCarley, RW
中科院分区：
文献类型：
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作者：
Basheer, R;Porkka-Heiskanen, T;McCarley, RW
在几个脑区中，细胞外腺苷（AD）水平在清醒期间高于睡眠，并且在长时间清醒期间，基底前脑中的AD水平逐渐增加。类似地，清醒时几个大脑区域的c-Fos水平高于睡眠，并且在长时间清醒期间保持升高。在本研究中，我们研究了细胞外AD水平对大鼠基底前脑c-Fos蛋白和激活蛋白1（AP 1）结合的影响。细胞外AD的水平增加诱导保持清醒的动物，或通过局部灌注AD到基底前脑。在长时间的觉醒期间，使用体内微透析监测细胞外AD浓度。采用多导睡眠图记录AD灌注对行为状态的影响。在灌注期结束时，分析基底前脑组织的c-Fos蛋白和AP 1结合水平。体内微透析测量显示，随着觉醒时间的延长，AD水平增加。单侧灌注AD（300 μ M）增加非REM睡眠和三角洲功率（0.5至4赫兹）相比，大鼠灌注人工脑脊液。c-Fos蛋白和API DNA结合的水平高，在基底前脑的睡眠剥夺的动物和灌注AD的动物。结果表明，AD可能介导，至少部分，通过诱导c-Fos蛋白和随后的AP 1结合的睡眠剥夺的长期影响。(C)1999 Elsevier Science B. V.保留所有权利。
In several brain areas, extracellular adenosine (AD) levels are higher during waking than sleep and during prolonged wakefulness AD levels in the basal forebrain increase progressively. Similarly, c-Fos levels in several brain areas are higher during waking than sleep and remain elevated during prolonged wakefulness. In the present study, we investigated the effect of extracellular AD levels on c-Fos protein and activator protein-1 (AP1) binding in the basal forebrain of rats. Increased levels of extracellular AD were induced either by keeping the animals awake, or by local perfusion of AD into the basal forebrain. During prolonged wakefulness extracellular AD concentration was monitored using in vivo microdialysis. The effect of AD perfusion on the behavioral states was recorded using polysomnography. At the end of the perfusion period the basal forebrain tissue was analyzed for the levels of c-Fos protein and AP1 binding. In vivo microdialysis measurements showed an increase in AD levels with prolonged wakefulness. Unilateral perfusion of AD (300 mu M) increased non-REM sleep and delta power (0.5 to 4 Hz) when compared to rats perfused with artificial CSF. The levels of c-Fos protein and the API DNA binding were high in the basal forebrain of both sleep-deprived animals and in animals perfused with AD. The results suggest that AD might mediate, at least in part, the long term effects of sleep deprivation by inducing c-Fos protein and subsequent AP1 binding. (C) 1999 Elsevier Science B.V. All rights reserved.