Binding of ATP to the progesterone receptor.
Binding of ATP to the progesterone receptor.
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ATP 与孕酮受体的结合。
DOI：
10.1073/pnas.72.3.901
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发表时间：
1975
影响因子：
11.1
通讯作者：
D. Toft
中科院分区：
文献类型：
--
作者：
V. Moudgil;D. Toft
孕激素受体复合物与核苷酸的可能相互作用进行了测试，通过亲和层析。用硫酸铵沉淀法部分纯化了鸡输卵管胞浆孕酮受体。当孕酮与受体结合时，所产生的复合物可选择性地吸附在ATP-Sepharose柱上。这种相互作用是可逆的，并且具有离子性质，因为它可以被高盐条件破坏。竞争性结合试验用于测试受体与几种其他核苷酸（包括ADP、AMP和cAMP）结合的特异性。从这些研究中可以明显看出结合ATP的明确特异性。当ATP被添加到受体制剂中时，核苷酸不影响受体的沉降特性或激素结合特性。虽然ATP的功能仍然未知，但这些研究表明这种核苷酸在激素受体活性的某些方面发挥作用。
The possible interaction of progesterone--receptor complexes with nucleotides was tested by affinity chromatography. The cytosol progesterone receptor from hen oviduct was partially purified by ammonium sulfate precipitation before use. When progesterone was bound to the receptor, the resulting complex could be selectively adsorbed onto columns of ATP-Sepharose. This interaction was reversible and of an ionic nature since it could be disrupted by high-salt conditions. A competitive binding assay was used to test the specificity of receptor binding to several other nucleotides, including ADP, AMP, and cAMP. A clear specificity for binding ATP was evident from these studies. When ATP was added to receptor preparations, the nucleotide did not affect the sedimentation properties or hormone binding characteristics of the receptor. Although the function of ATP remains unknown, these studies indicate a role of this nucleotide in some aspect of hormone receptor activity.