Changes in CD of hyaluronates and chondroitins upon periodate oxidation.
Changes in CD of hyaluronates and chondroitins upon periodate oxidation.
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高碘酸盐氧化后透明质酸盐和软骨素 CD 的变化。
DOI：
10.1002/bip.360260815
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Chakrabarti,B
中科院分区：
文献类型：
--
作者：
Ueno,N;Chakrabarti,B
本文用圆二色谱研究了高碘酸氧化后透明质酸和硫酸软骨素的构象变化。我们监测了这些聚合物在4°C下在pH 5.6的缓冲液中被高碘酸逐渐氧化。透明质酸盐在208 nm处的负CD带在氧化后强度降低，并在氧化16 h后改变其符号。相比之下，硫酸软骨素的208 nm CD谱带减少，但即使在氧化48 h后也没有显示符号变化。透明质酸盐和软骨素之间溶液构象的特定差异可能是氧化诱导二色性行为差异的原因。这些聚合物的可用的X射线衍射分析的结果进行了讨论。
Changes in conformation of hyaluronate and chondroitin sulfates following periodate oxidation were studied by CD. We monitored the progressive oxidation of these polymers by periodic acid at 4°C in pH 5.6 buffer. The negative CD band of hyaluronate at 208 nm decreased in intensity upon oxidation and changed its sign after 16 h of oxidation. In contrast, the 208‐nm CD band of chondroitin sulfates decreased, but showed no change in sign even after 48 h of oxidation. A specific difference in solution conformation between hyaluronate and chondroitins may be responsible for the difference in oxidation‐induced dichroic behavior. The results are discussed in terms of available x‐ray diffraction analyses of these polymers.