Interaction of E. coli G Factor with the 50S Ribosomal Subunit
Interaction of E. coli G Factor with the 50S Ribosomal Subunit
复制标题
大肠杆菌 G 因子与 50S 核糖体亚基的相互作用
作者：
J. Bodley;Lillian Lin
转位，即核糖体沿mRNA的运动，是一个活跃的过程，它需要GTP的水解和可溶性蛋白G因子1-5的参与。6，7发现了转位的抗生素抑制剂夫西地酸稳定了涉及核糖体、G因子和GDP的复合体，为研究这些参与者之间的相互作用提供了一个新的强大的工具，并至少间接地了解了这一步在蛋白质合成中的机制。
TRANSLOCATION, the movement of the ribosome along mRNA, is an active process which requires the hydrolysis of GTP and the participation of the soluble protein, G factor1–5. The discovery6,7 that fusidic acid, an antibiotic inhibitor of translocation, stabilizes a, complex involving ribosomes, G factor, and GDP provides a new and powerful tool with which to investigate the interaction among these participants in translocation and to provide at least indirect insight into the mechanism of this step in protein synthesis.