A comparison of human tumour-cell clonogenicity in methylcellulose and agar culture.
A comparison of human tumour-cell clonogenicity in methylcellulose and agar culture.
复制标题
甲基纤维素和琼脂培养物中人类肿瘤细胞克隆形成的比较。
DOI：
10.1038/bjc.1980.343
复制
发表时间：
1980
影响因子：
8.8
通讯作者：
Fry,SE
中科院分区：
文献类型：
--
作者：
Buick,RN;Fry,SE
能够肿瘤再增殖的肿瘤细胞（肿瘤干细胞）是决定人类肿瘤生物学反应的关键细胞（Steel，1977）。由于原位测定法对于人肿瘤再增殖细胞是不可行的，因此测量半固体培养物中的克隆形成性作为肿瘤干细胞群体的近似值（Hamburger & Salmon，1977; Hamburger et al. 1978年; Buick等人，1979 a; Salmon等人，1978年）。半固体支持物是确定培养物集落形成性的一个可能变量，如果研究者希望随后去除集落用于进一步研究，则也是一个重要的考虑因素。使用甲基纤维素和软琼脂的组合的技术已经证明在过渡细胞癌的培养中是成功的（Buick等人，1979 a），并且单独的甲基纤维素已被证明是用于评估的有用的半固体支持物（Buick等人，1977）和随后的操作（Buick等人，1979 b）的肿瘤克隆形成细胞在人类急性髓性白血病。在这里，我们报告的研究结果比较培养克隆形成的特点，甲基纤维素和琼脂的肿瘤细胞来源于24个恶性积液转移性卵巢癌或乳腺癌患者。恶性渗出液来自在亚利桑那健康科学中心（Pts 1-11）和安大略癌症研究所（Pts
NEOPLASTIC CELLS capable of tumour repopulation (tumour stem cells) are the critical cells in determining biological responses of human tumours (Steel, 1977). Since no in situ assay is feasible for human tumour-repopulating cells, clonogenicity in semi-solid culture is measured as an approximation of the tumour stem-cell population (Hamburger & Salmon, 1977; Hamburger et al., 1978; Buick et al., 1979a; Salmon et al., 1978). Semi-solid support is one possible variable in the determination of culture clonogenicity, and is also an important consideration if the investigator wishes to subsequently remove colonies for further studies. Techniques using a combination of methylcellu-lose and soft agar have proved successful in the culture of transitional-cell carcinoma (Buick et al., 1979a) and methylcellulose alone has proved to be a useful semisolid support for the assessment (Buick et al., 1977) and subsequent manipulation (Buick et al., 1979b) of tumour clonogenic cells in human acute myeloblastic leukaemia.Here we report the results of a study comparing the characteristics of culture clonogenicity in methylcellulose and agar of tumour cells derived from 24 malignant effusions from patients with metastatic ovarian or breast carcinoma. Malignant effusions were obtained from cancer patients undergoing routine clinical care at the Arizona Health Sciences Center (Pts 1-11) and Ontario Cancer Institute (Pts