Vaccination of calves with Mycobacteria bovis Bacilli Calmete Guerin (BCG) induced rapid increase in the proportion of peripheral blood γδ T cells
Vaccination of calves with Mycobacteria bovis Bacilli Calmete Guerin (BCG) induced rapid increase in the proportion of peripheral blood γδ T cells
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DOI：
10.1016/j.vetimm.2008.12.021
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发表时间：
2009-08-15
影响因子：
1.8
通讯作者：
Ameni, Gobena
中科院分区：
文献类型：
--
作者：
Buza, Joram;Kiros, Tadele;Ameni, Gobena
研究了皮下接种牛分枝杆菌卡介苗后外周血T细胞亚群比例的变化。犊牛注射约8 × 10(6)个卡介苗杆菌，每隔一周采集血液样本进行流式细胞术分析，以确定CD4（+）、CD8（+）和γ δ T细胞的比例。此外，体外用牛支原体纯化蛋白衍生物（PPD）刺激全血样品，并用ELISA法定量测定分泌的ifn - γ。结果显示细胞和细胞因子的变化可分为三个阶段。第一阶段发生在接种疫苗后的前2周内，WC1(+) γ δ T细胞比例增加，同时ifn - γ分泌增加。这两种反应在2周时达到顶峰，随后减弱。第二阶段涉及CD4/CD8比率的增加，这是CD4(+) T细胞比例在4至6周之间增加的结果。在第三阶段，由于CD8(+) T细胞的比例在8至10周之间增加，CD4/CD8比率下降。令人惊讶的是，ifn - γ反应与γ δ而不是CD4（+）或CD8(+) T细胞的变化有关，这表明这种细胞因子是由γ δ T细胞分泌的。这些结果与报道的伽马δ T细胞快速起作用并桥接先天和经典适应性免疫反应的能力一致。(C) 2009 Elsevier B.V.版权所有
Changes in the proportion of peripheral blood T cell subsets after subcutaneous inoculation of cattle with Mycobacterium bovis Bacille Calmette-Guerin (BCG) were studied. Calves were injected with approximately 8 x 10(6) BCG bacillus and blood samples collected at weekly intervals for flow-cytometric analyses to determine the proportion of CD4(+), CD8(+) and gamma delta T cells. In addition, whole blood samples were stimulated in vitro with M. bovis purified protein derivative (PPD) and the secreted IFN-gamma quantified by ELISA. Results showed cellular and cytokine changes which could be categorized into three phases. The first phase occurred within the first 2 weeks after vaccination involving an increase in proportion of WC1(+) gamma delta T cells and a concomitant increase in the secretion of IFN-gamma. These two responses peaked at 2 weeks and waned thereafter. The second phase involved an increase in the CD4/CD8 ratio as a result of an increase in the proportion of CD4(+) T cells between 4 and 6 weeks. The third phase involved a decrease in the CD4/CD8 ratio due to an increase in the proportion of CD8(+) T cells between 8 and 10 weeks. Surprisingly, the IFN-gamma response was associated with changes in the gamma delta rather than the CD4(+) or CD8(+) T cells, suggesting that this cytokine was secreted by gamma delta-T cells. These results are consistent with the reported ability of gamma delta T cells to act rapidly and bridging the innate and classically adaptive immune responses. (C) 2009 Elsevier B.V. All rights reserved.