DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE.
DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE.
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DOI：
10.1084/jem.120.2.121
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发表时间：
1964-08-01
期刊：
影响因子：
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通讯作者：
ROWE WP
中科院分区：
文献类型：
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作者：
POPE JH;ROWE WP
用免疫荧光技术，涉及使用血清的仓鼠与SV 40肿瘤，核荧光检测在每五个细胞系，来自四个哺乳动物物种，转化的SV 40病毒。基本上所有的细胞核，包括多核细胞的细胞核，都是荧光染色的。携带SV 40肿瘤的仓鼠的血清也被发现在用SV 40病毒急性感染的敏感细胞（AGMK或BSC-1）中产生核荧光。这些发现提供了进一步的证据，即细胞掺入SV 40病毒基因组，通过合成至少一种病毒特异性抗原部分表达基因组，是所有SV 40转化细胞的整体性质。
With an immunofluorescent technique involving the use of serum of hamsters with SV40 tumors, nuclear fluorescence was detected in each of five cell lines, derived from four mammalian species, transformed by SV40 virus. Essentially all nuclei, including those of multinuclear cells, were fluorescent-stainable. Serum of hamsters bearing SV40 tumors was also found to give nuclear fluorescence in susceptible cells (AGMK or BSC-1) acutely infected with SV40 virus. These findings provide further evidence that cellular incorporation of the SV40 viral genome, with partial expression of the genome by synthesis of at least one virus-specific antigen, is an integral property of all SV40 transformed cells.