Cloning and Expression of Guanylin‐like Peptides in Teleost Fish
Cloning and Expression of Guanylin‐like Peptides in Teleost Fish
复制标题
硬骨鱼类鸟苷酸肽的克隆与表达
DOI：
10.1196/annals.1327.042
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发表时间：
2005
影响因子：
5.2
通讯作者：
Gillian D. Wilson
中科院分区：
文献类型：
--
作者：
G. Cramb;Anne;I. McWilliam;Gillian D. Wilson
摘要：鸟苷肽家族的同源物在硬骨鱼中表达。使用鳗鱼作为模型广盐物种，克隆了三种肽（鸟苷蛋白、尿鸟苷蛋白和雷诺鸟苷蛋白）的 cDNA，并发现它们在肾和肠上皮细胞中表达。海水（SW）驯化导致未成熟的“黄”鳗和性成熟的“银”鳗的肠上皮细胞中尿鸟苷蛋白mRNA表达上调。 SW驯化还导致银鳗肠道中鸟苷蛋白mRNA的增加，而在任何条件下雷诺鸟苷蛋白mRNA的表达都没有变化。 SW 适应后肾脏中没有发现表达变化。
Abstract: Homologues of the guanylin peptide family are expressed in teleost fish. Using the eel as a model euryhaline species, cDNAs for three peptides, guanylin, uroguanylin, and renoguanylin, were cloned and found to be expressed within both renal and intestinal epithelia. Seawater (SW) acclimation resulted in upregulation of uroguanylin mRNA expression in the intestinal epithelia of both immature “yellow” and sexually mature “silver” eels. SW acclimation also resulted in an increase in guanylin mRNA in silver eel intestine, whereas there was no change in renoguanylin mRNA expression under any condition. No changes in expression were found in the kidney following SW acclimation.