GROWTH-STIMULATION OF A HUMAN COLORECTAL-CARCINOMA CELL-LINE BY INTERLEUKIN-1 AND INTERLEUKIN-6 AND ANTAGONISTIC EFFECTS OF TRANSFORMING GROWTH FACTOR-BETA-1
GROWTH-STIMULATION OF A HUMAN COLORECTAL-CARCINOMA CELL-LINE BY INTERLEUKIN-1 AND INTERLEUKIN-6 AND ANTAGONISTIC EFFECTS OF TRANSFORMING GROWTH FACTOR-BETA-1
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DOI：
10.1016/0959-8049(92)90031-v
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发表时间：
1992-01-01
影响因子：
8.4
通讯作者：
ODARTCHENKO, N
中科院分区：
文献类型：
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作者：
LAHM, H;PETRALMALEC, D;ODARTCHENKO, N
我们分析了白细胞介素-1 (IL-1)、IL-6 和转化生长因子-β-1 (TGF-β-1) 对八种结直肠癌细胞系生长的影响。 IL-1 刺激两个细胞系（LS411N 和 LS1034）的生长高达 20 倍，IL-6 增强 LS1034 的增殖超过 5 倍。两种细胞因子还增强了甲基纤维素中 LS1034 的集落形成。在两种生长条件下，IL-1 都是最有效的刺激剂。然而，在 IL-1 中添加 IL-6 可协同增强单层培养物中 LS1034 的增殖，并额外增加甲基纤维素中形成的集落数量。此外，TGF-β-1 强烈降低了 LS1034 的生长速度。低量的 TGF-β-1 显着抑制 LS1034 对 IL-1 的反应，并完全消除 IL-6 诱导的增殖。我们得出的结论是，不同的细胞因子可以为调节结直肠癌细胞的生长提供不同的信号。
We analysed the effect of interleukin-1 (IL-1), IL-6 and transforming growth factor-beta-1 (TGF-beta-1) on the growth of a panel of eight colorectal carcinoma cell lines. IL-1 stimulated growth of two lines (LS411N and LS1034) up to 20-fold and IL-6 enhanced proliferation of LS1034 more than 5-fold. Both cytokines also augmented colony-formation of LS1034 in methylcellulose. Under both growth conditions IL-1 was the most potent stimulator. However, the addition of IL-6 to IL-1 synergistically enhanced proliferation of LS1034 in monolayer culture and additively augmented the number of colonies formed in methylcellulose. Furthermore, TGF-beta-1 strongly reduced the growth rate of LS1034. Low amounts of TGF-beta-1 markedly inhibited the response of LS1034 to IL-1 and totally abrogated proliferation induced by IL-6. We conclude that different cytokines can provide distinct signals for the regulation of growth of colorectal carcinoma cells.