Mutual potentiation of plant immunity by cell-surface and intracellular receptors
Mutual potentiation of plant immunity by cell-surface and intracellular receptors
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DOI：
10.1038/s41586-021-03315-7
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发表时间：
2021-03-10
期刊：
影响因子：
64.8
通讯作者：
Jones, Jonathan D. G.
中科院分区：
文献类型：
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作者：
Ngou, Bruno Pok Man;Ahn, Hee-Kyung;Jones, Jonathan D. G.
植物免疫系统涉及检测细胞间病原体衍生分子的细胞表面受体，以及在检测到在植物细胞内起作用的病原体分泌的效应蛋白时激活免疫的细胞内受体。由表面受体介导的免疫已被广泛研究（1），但在缺乏表面受体介导的免疫的情况下，由细胞内受体介导的免疫很少被研究。此外，这两种免疫途径之间的相互作用知之甚少。在这里，通过激活细胞内受体而不诱导表面受体介导的免疫，我们分析了这两个不同的免疫系统在拟南芥之间的相互作用。病原体识别表面受体激活多种蛋白激酶和NADPH氧化酶，我们发现，细胞内受体主要增强这些蛋白质的激活，通过增加其丰度通过几种机制。同样，依赖于细胞内受体的过敏反应通过表面受体的激活而强烈增强。单独激活任一免疫系统都不足以提供针对细菌病原体假单胞菌的有效抗性。因此，植物中细胞表面和细胞内受体激活的免疫途径相互增强，以激活对病原体的强大防御。这些发现重塑了我们对植物免疫的理解，并对作物改良产生了广泛的影响。
The plant immune system involves cell-surface receptors that detect intercellular pathogen-derived molecules, and intracellular receptors that activate immunity upon detection of pathogen-secreted effector proteins that act inside the plant cell. Immunity mediated by surface receptors has been extensively studied(1), but that mediated by intracellular receptors has rarely been investigated in the absence of surface-receptor-mediated immunity. Furthermore, interactions between these two immune pathways are poorly understood. Here, by activating intracellular receptors without inducing surface-receptor-mediated immunity, we analyse interactions between these two distinct immune systems in Arabidopsis. Pathogen recognition by surface receptors activates multiple protein kinases and NADPH oxidases, and we find that intracellular receptors primarily potentiate the activation of these proteins by increasing their abundance through several mechanisms. Likewise, the hypersensitive response that depends on intracellular receptors is strongly enhanced by the activation of surface receptors. Activation of either immune system alone is insufficient to provide effective resistance against the bacterial pathogen Pseudomonas syringae. Thus, immune pathways activated by cell-surface and intracellular receptors in plants mutually potentiate to activate strong defences against pathogens. These findings reshape our understanding of plant immunity and have broad implications for crop improvement.