Left-right asymmetric cell intercalation drives directional collective cell movement in epithelial morphogenesis.
Left-right asymmetric cell intercalation drives directional collective cell movement in epithelial morphogenesis.
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DOI：
10.1038/ncomms10074
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发表时间：
2015-12-10
影响因子：
16.6
通讯作者：
Kuranaga E
中科院分区：
文献类型：
--
作者：
Sato K;Hiraiwa T;Maekawa E;Isomura A;Shibata T;Kuranaga E
形态发生上皮运动发生在胚胎发生过程中并驱动复杂的组织形成。然而，上皮细胞如何在保持上皮完整性的同时协调其单向运动尚不清楚。在这里，我们提出了一种基于果蝇生殖器旋转的集体上皮细胞运动的新机制，其中上皮组织围绕生殖器顺时针旋转。我们发现这种细胞运动是自主发生的并且需要肌球蛋白 II。移动细胞表现出重复的左右偏向连接重塑，同时保持与相邻细胞的粘附，与极化肌球蛋白 II 分布相关。减少肌球蛋白 ID（已知会导致上皮组织逆时针运动）可逆转肌球蛋白 II 的分布。数值模拟表明，细胞嵌入的左右不对称足以诱导单向细胞运动。细胞运动方向也与平面细胞形状手性相关。这些发现支持一个模型，其中上皮片内的左右不对称细胞嵌入驱动集体细胞向同一方向运动。胚胎发生过程中协调的上皮运动驱动复杂的组织形成，但如何协调这种运动以维持上皮的完整性尚不清楚。在这里，作者表明，细胞嵌入的左右不对称驱动果蝇生殖器发育中上皮细胞的顺时针旋转。
Morphogenetic epithelial movement occurs during embryogenesis and drives complex tissue formation. However, how epithelial cells coordinate their unidirectional movement while maintaining epithelial integrity is unclear. Here we propose a novel mechanism for collective epithelial cell movement based on Drosophila genitalia rotation, in which epithelial tissue rotates clockwise around the genitalia. We found that this cell movement occurs autonomously and requires myosin II. The moving cells exhibit repeated left–right-biased junction remodelling, while maintaining adhesion with their neighbours, in association with a polarized myosin II distribution. Reducing myosinID, known to cause counter-clockwise epithelial-tissue movement, reverses the myosin II distribution. Numerical simulations revealed that a left–right asymmetry in cell intercalation is sufficient to induce unidirectional cellular movement. The cellular movement direction is also associated with planar cell-shape chirality. These findings support a model in which left–right asymmetric cell intercalation within an epithelial sheet drives collective cellular movement in the same direction. Coordinated epithelial movement during embryogenesis drives complex tissue formation, but how this movement is coordinated to maintain epithelial integrity is not clear. Here the authors show that left-right asymmetry in cell intercalation drives clockwise rotation of epithelia in Drosophila genital development.