Application of hydroxyapatite nanoparticle-assembled powder using basic fibroblast growth factor as a pulp-capping agent
Application of hydroxyapatite nanoparticle-assembled powder using basic fibroblast growth factor as a pulp-capping agent
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DOI：
10.4012/dmj.2018-198
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发表时间：
2019-10-01
影响因子：
2.5
通讯作者：
Yamamoto, Kazuyo
中科院分区：
文献类型：
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作者：
Imura, Kazuki;Hashimoto, Yoshiya;Yamamoto, Kazuyo
我们以前制造的羟基磷灰石（HAP）纳米粒子组装粉末（纳米HAP）板和颗粒组装低结晶度的HAP纳米粒子没有模板/粘合剂分子或高温/压力处理。在这项研究中，我们将纳米HAP与促进成牙本质细胞分化的成纤维细胞生长因子（FGF）2相结合，并将其用作大鼠磨牙牙本质缺损的盖髓剂。然后在加盖后1周和2周对组织反应进行放射学和组织学评估，以评估该材料的生物相容性和促进硬组织形成的能力。应用nano-HAP/FGF 2诱导牙髓细胞和血管的侵袭，并且一致地发现刺激包含许多牙本质小管的牙本质桥的形成。因此，我们成功地通过使用生理方法来治疗牙髓暴露，以促进组织再生。应该进行进一步的研究，以确切地解释纳米HAP/FGF 2组合如何有助于钙化组织的形成。
We have previously fabricated hydroxyapatite (HAP) nanoparticle-assembled powder (nano-HAP) plates and granules by assembling low-crystallinity HAP nanoparticles without template/binder molecules or high-temperature/pressure treatments. In this study, we combined the nano-HAP with fibroblast growth factor (FGF) 2, which promotes odontoblast differentiation, and used this as a pulp-capping agent for dentin defects created in rat molars. The tissue response was then radiologically and histologically assessed at 1 and 2 weeks after capping, to assess the biocompatibility and ability of this material to promote hard tissue formation. The application of nano-HAP/FGF2 induced the invasion of dental pulp cells and vessels, and was consistently found to stimulate formation of a dentinal bridge containing numerous dentinal tubules. We thus succeeded in treating the pulp exposure by using a physiological approach to promote tissue regeneration. Further investigations should be performed to explain exactly how the nano-HAP/FGF2 combination contributes to calcified tissue formation.