Further characterization of an interleukin-2-like cytokine produced by Xenopus laevis T lymphocytes.
Further characterization of an interleukin-2-like cytokine produced by Xenopus laevis T lymphocytes.
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非洲爪蟾 T 淋巴细胞产生的白细胞介素 2 样细胞因子的进一步表征。
DOI：
10.1155/1993/68197
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Cohen,N
中科院分区：
文献类型：
--
作者：
Haynes,L;Cohen,N
T细胞生长因子(TCGF)是由抗原或有丝分裂原刺激的南非爪蛙T淋巴细胞产生的。这项研究进一步确定了这种细胞因子的物理和生物学特性，并证明TCGF在生物化学上类似于哺乳动物的白介素2(IL-2)。从SDS-PAGE上洗脱的生物活性TCGF显示分子量为16kD，凝集素亲和层析表明TCGF上的碳水化合物与人IL-2上的糖的三维构型相似。T细胞有丝分裂原植物血凝素(PHA)刺激脾细胞1天后即可检测到TCGF的分泌，刺激2~3天后达到高峰。最后，尽管爪哇TCGF和哺乳动物IL-2在生物学和物理上有相似之处，但抗人IL-2单抗不能识别爪哇TCGF。
A T‐cell growth factor (TCGF) is produced by antigen‐ or mitogen‐stimulated T lymphocytes from the South African clawed frogXenopus laevis. This study further defines the physical and biological properties of this cytokine and demonstrates that TCGF is biochemically similar to mammalian interleukin‐2 (IL‐2). Biologically active TCGF eluted from SDS‐PAGE displays a Mrof 16 kD and lectin‐affinity chromatography indicates that the three‐dimensionmal configuration of carbohydrates on TCGF and human IL‐2 is similar. Secretion of TCGF is detectable 1 day after stimulation of splenocytes with the T‐cell mitogen phytohemagglutinin (PHA) and peaks following 2 to 3 days of stimulation. Finally, despite the biological and physical similarities betweenXenopusTCGF and mammalian IL‐2, anti‐human IL‐2 monoclonal antibodies do not recognizeXenopusTCGF.