Extraction of soil organic phosphorus.
Extraction of soil organic phosphorus.
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DOI：
10.1016/j.talanta.2004.11.012
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发表时间：
2005-04
期刊：
影响因子：
6.1
通讯作者：
Benjamin L Turner;B. Cade‐Menun;L. Condron;S. Newman
中科院分区：
文献类型：
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作者：
Benjamin L Turner;B. Cade‐Menun;L. Condron;S. Newman
有机磷是土壤生态地球化学循环的重要组成部分，但在分析前必须从土壤中提取。在这里，我们批判性地回顾了土壤有机磷的提取，包括定量，形态和生物有效性的评估程序。定量萃取通常需要强酸和强碱，这不可避免地改变了化学结构。然而，涉及氢氧化钠和EDTA（乙二胺四乙酸盐）的单步程序适用于大多数土壤，并有助于随后通过核磁共振光谱进行形态分析。在所有程序中，用比色法分析提取物是一个潜在的误差来源，因为在无机多磷酸盐或无机磷酸盐与腐殖物质之间的复合物存在下，有机磷被高估。顺序提取方案基于化学溶解度来提取有机磷，但与潜在生物利用度的联系是误导性的。研究应迫切致力于建立具有生态相关性的土壤有机磷可提取库。
Organic phosphorus is an important component of soil biogeochemical cycles, but must be extracted from soil prior to analysis. Here we critically review the extraction of soil organic phosphorus, including procedures for quantification, speciation, and assessment of biological availability. Quantitative extraction conventionally requires strong acids and bases, which inevitably alter chemical structure. However, a single-step procedure involving sodium hydroxide and EDTA (ethylenediaminetetraacetate) is suitable for most soils and facilitates subsequent speciation by nuclear magnetic resonance spectroscopy. Analysis of extracts by molybdate colorimetry is a potential source of error in all procedures, because organic phosphorus is overestimated in the presence of inorganic polyphosphates or complexes between inorganic phosphate and humic substances. Sequential extraction schemes fractionate organic phosphorus based on chemical solubility, but the link to potential bioavailability is misleading. Research should be directed urgently towards establishing extractable pools of soil organic phosphorus with ecological relevance.