Limited IgE cross-reactivity between Dermatophagoides pteronyssinus and Glycyphagus domesticus in patients naturally exposed to both mite species
Limited IgE cross-reactivity between Dermatophagoides pteronyssinus and Glycyphagus domesticus in patients naturally exposed to both mite species
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DOI：
10.1016/j.jaci.2007.02.028
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发表时间：
2007-07-01
影响因子：
14.2
通讯作者：
Barber, Domingo
中科院分区：
文献类型：
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作者：
Arias-Irigoyen, Jose;Lombardero, Manuel;Barber, Domingo
背景：在分析焦线螨类和储藏类蠕形螨之间的IgE交叉反应以及它们作为真正致敏剂的作用时，发现了相互矛盾的结果。目的：我们试图研究自然暴露于这种蠕形螨以及更普遍的屋尘螨的患者中对家蝇的敏感率以及它们之间的IgE交叉反应。方法：通过光学显微镜检测连续来自西班牙西南部Huelva的蠕形螨过敏患者的性欲样本。采用皮肤刺激法和结膜刺激法评价临床致敏性。采用血清IgE测定、RAST抑制试验、IgE免疫印迹试验和尘螨第2组免疫分析等方法，研究了屋尘在IgE水平上的交叉反应。结果：在约95%的屋尘样本中存在屋尘螨之后，最重要的是家蝇，约50%的样本中存在屋尘螨。腐食酪菌和破坏鳞毛虫分别位居第三和第四位。在家中患有革兰氏阴性杆菌的患者中，约有一半对这种螨类致敏。屋尘螨和家蝇之间存在较低的IgE交叉反应，而在第二组变应原水平上的嗜甘虫性粉尘中检测到重要的IgE交叉反应。结论：在自然暴露于甘膦粉尘的患者中，甘膦粉尘可以作为独立于焦甘膦粉尘的主要致敏剂。临床意义：在有足够的粉尘生长条件的地区，在诊断单元中加入甘膦粉尘提取物对于确保正确的诊断是重要的。
Background: Contradictory results have been found when analyzing the IgE cross-reactivity among pyroglyphid mites and storage mites, as well as the role of these as true sensitizing agents.Objective: We sought to study the prevalence of sensitization to Glycyphagus domesticus in patients naturally exposed to this mite together with the more ubiquitous Dermatophagoides pteronyssinus and the IgE cross-reactivity between them.Methods: Mite species present in house (lust samples of consecutive patients with mite allergy from Huelva (southwest Spain) were determined by means of light microscopy. Clinical sensitization was assessed by using skin prick and conjunctival provocation tests. Cross-reactivity at the IgE level was studied by using serum IgE determination and inhibition of RAST, IgE immunoblotting, and mite group 2 immunoassays.Results: After D pteronyssinus, which is present in about 95% of house dust samples, G domesticus was the most important mite and present in about 50% of the samples. Tyrophagus putrescentiae and Lepidoglyphus destructor were detected in third and fourth place, respectively. About half of the patients with G domesticus at home were sensitized to this mite. A low IgE cross-reactivity was observed between D pteronyssinus and G domesticus, but an important IgE cross-reactivity was detected among glycyphagid mites at the level of group 2 allergens. Conclusion: Glycyphagid mites can act as primary sensitizing agents independently of pyroglyphid mites in a subset of patients naturally exposed to them.Clinical implications: The inclusion of glycyphagid mite extracts in the diagnostic battery in areas with adequate mite growing conditions is important to ensure proper diagnosis.