A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.

A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
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DOI:
10.1083/jcb.70.3.581
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发表时间:
1976-09
期刊:
The Journal of cell biology
影响因子:
--
通讯作者:
Blobel G
Blobel G
中科院分区:
其他
文献类型:
--
作者:
Dwyer N;Blobel G

文献摘要

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本文介绍了一种从大鼠肝细胞核中分离核孔复合体--板层部分的改进方法。有证据表明,150-A厚的蛋白质结构的孤立的板层与内核膜相对,连接核孔复合体并包围整个核。
A modified procedure for the isolation of a nuclear pore complex-lamina fraction from rat liver nuclei is described. Evidence is provided that the isolated lamina, a 150-A thick, proteinaceous structure, apposes the inner nuclear envelope membrane, connecting nuclear pore complexes and surrounding the entire nucleus.