Nucleotide Sequence Analysis of the Polyhedrin Gene of Antheraea pernyi Nucleopolyhedrovirus and Construction of a Transfer Vector Plasmid
Nucleotide Sequence Analysis of the Polyhedrin Gene of Antheraea pernyi Nucleopolyhedrovirus and Construction of a Transfer Vector Plasmid
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柞蚕核多角体病毒多角体蛋白基因核苷酸序列分析及转移载体质粒的构建
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
S. Miyajima
中科院分区：
文献类型：
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作者：
J. Kobayashi;R. Ando;W. Ying;H. Jiao;S. Miyajima
The polyhedrin gene of Antheraea Pernyi nucleopolyhedrovirus (AnpeNPV) was identified in a 6. 6 kbp Pstl fragment of the viral DNA genome by Southern blot hybridization using the polyhedrin gene of Bombyx mori NPV (BmNPV) as a probe. The nucleotide sequence analysis revealed that the AnpeNPV polyhedrin gene contained a promoter sequence of 51 bp and a coding sequence of 738 bp encoding 245 amino acids. Amino acid sequence comparison was made with both polyhedrins and granulins of other baculoviruses and very high homologies were detected with members of the group 1 NPV. Among them a NPV of another saturuniid moth, Attacus ricini (一 Samia cynthia ricinz) showed the highest homology, indicating that the two NPVs are closely related or variants. For the establishment of a novel baculovirus expression vector system using AnpeNPV and diapausing pupae of A. Pernyi, we have constructed a transfer vector plasmid pApCHI, which enable the foreign gene expression under the control of the strong AnpeNPV polyhedrin promoter.