Analysing the evolutional and functional differentiation of four types of Daphnia magna cryptochrome in Drosophila circadian clock
Analysing the evolutional and functional differentiation of four types of Daphnia magna cryptochrome in Drosophila circadian clock
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DOI：
10.1038/s41598-019-45410-w
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发表时间：
2019-06
影响因子：
4.6
通讯作者：
Yohei Nitta;Sayaka Matsui;Y. Kato;Yosuke Kaga;Kenkichi Sugimoto;Atsushi Sugie
中科院分区：
文献类型：
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作者：
Yohei Nitta;Sayaka Matsui;Y. Kato;Yosuke Kaga;Kenkichi Sugimoto;Atsushi Sugie
隐花色素（CRY）在生物钟的输入中起着重要的作用，但在每个物种中的基因拷贝是进化上不同的。I型受体在中央时钟中作为光感受器分子发挥作用，而II型受体直接调节时钟蛋白的转录活性。其他类型的动物基因在分子钟中的功能仍然未知。水蚤Daphnia magnagonacontainsfourCrygenes.然而，分析这四个基因仍然很困难。在本研究中，我们利用果蝇强大的遗传资源来研究两个物种之间CRY蛋白的进化和功能差异。我们报告的差异，在亚细胞定位的eachD。magnaCRY蛋白在果蝇的钟状神经元中的表达。昼夜节律行为实验表明，D.在果蝇的分子钟中，magnaB在功能上并不保守。这些发现提供了一个新的角度对进化保守的CRY，作为功能的fourD。magnaCRY蛋白具有不同的亚细胞定位水平。此外，D.在进化上已经与果蝇不同。这项研究强调了物种之间存在的广泛的功能多样性，在他们的互补ofCrygenes。
Cryptochrome (CRY) plays an important role in the input of circadian clocks in various species, but gene copies in each species are evolutionarily divergent. Type I CRYs function as a photoreceptor molecule in the central clock, whereas type II CRYs directly regulate the transcriptional activity of clock proteins. Functions of other types of animal CRYs in the molecular clock remain unknown. The water fleaDaphnia magnacontains fourCrygenes. However, it is still difficult to analyse these four genes. In this study, we took advantage of powerful genetic resources available fromDrosophilato investigate evolutionary and functional differentiation of CRY proteins between the two species. We report differences in subcellular localisation of eachD. magnaCRY protein when expressed in theDrosophilaclock neuron. Circadian rhythm behavioural experiments revealed thatD. magnaCRYs are not functionally conserved in theDrosophilamolecular clock. These findings provide a new perspective on the evolutionary conservation of CRY, as functions of the fourD. magnaCRY proteins have diverse subcellular localisation levels. Furthermore, molecular clocks ofD. magnahave been evolutionarily differentiated from those ofDrosophila. This study highlights the extensive functional diversity existing among species in their complement ofCrygenes.