Lysine crotonylation is widespread on proteins of diverse functions and localizations in Toxoplasma gondii
Lysine crotonylation is widespread on proteins of diverse functions and localizations in Toxoplasma gondii
复制标题
赖氨酸巴豆酰化广泛存在于弓形虫不同功能和定位的蛋白质上
DOI：
10.1007/s00436-021-07057-3
复制
发表时间：
2021-03
影响因子：
2
通讯作者：
Zhu Xingquan
中科院分区：
文献类型：
--
作者：
Li Facai;Nie Lanbi;Elsheikha Hany M.;Yin Fangyuan;Zhu Xingquan
赖氨酸巴豆酰化（Kcr）是组蛋白上进化上保守的翻译后修饰（PTM）。然而，有关Kcr及其参与弓形虫生物学和代谢的信息有限。在本研究中，使用LC-MS/MS结合免疫亲和法进行了全球Kcr蛋白质组分析。共鉴定了分布在2719个巴豆酰化蛋白上的12，152个Kcr位点。与顶复门中赖氨酸乙酰化和琥珀酰化一致，Kcr与多种代谢途径相关，包括碳代谢、嘧啶代谢、糖酵解、异生和蛋白酶体。值得注意的是，许多与阶段转变相关的阶段特异性蛋白、组蛋白和组蛋白修饰酶都被发现具有Kcr位点，这表明Kcr可能参与了寄生虫阶段转变。顶端分泌细胞器的大部分成分被鉴定为与T.刚地。这些结果扩展了我们对Kcr蛋白质组的理解，并为进一步研究Kcr在T.弓形虫感染
Lysine crotonylation (Kcr) is an evolutionally conserved post-translational modification (PTM) on histone proteins. However, information about Kcr and its involvement in the biology and metabolism of Toxoplasma gondii is limited. In the present study, a global Kcr proteome analysis using LC-MS/MS in combination with immune-affinity method was performed. A total of 12,152 Kcr sites distributed over 2719 crotonylated proteins were identified. Consistent with lysine acetylation and succinylation in Apicomplexa, Kcr was associated with various metabolic pathways, including carbon metabolism, pyrimidine metabolism, glycolysis, gluconeogenesis, and proteasome. Markedly, many stage-specific proteins, histones, and histone-modifying enzymes related to the stage transition were found to have Kcr sites, suggesting a potential involvement of Kcr in the parasite stage transformation. Most components of the apical secretory organelles were identified as crotonylated proteins which were associated with the attachment, invasion, and replication of T. gondii. These results expanded our understanding of Kcr proteome and proposed new hypotheses for further research of the Kcr roles in the pathobiology of T. gondii infection.
登录
查看更多内容
约氏疟原虫 ApiAP2 基因的系统 CRISPR-Cas9 介导修饰揭示了寄生虫发育的功能见解
影响因子：
6.4
作者：
Cui Zhang;Zhenkui Li;Huiting Cui;Yuanyuan Jiang;Zhenke Yang;Xu Wang;Han Gao;Cong Liu;Shujia Zhang;Xin-zhuan Su;Jing Yuan
通讯作者：
Jing Yuan
影响因子：
3.2
作者：
通讯作者：
--
影响因子：
4.6
作者：
Cobbold SA;Santos JM;Ochoa A;Perlman DH;Llinás M
通讯作者：
Llinás M
DOI：
10.1084/jem.20101660
发表时间：
2011-07-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Yamamoto M;Ma JS;Mueller C;Kamiyama N;Saiga H;Kubo E;Kimura T;Okamoto T;Okuyama M;Kayama H;Nagamune K;Takashima S;Matsuura Y;Soldati-Favre D;Takeda K
通讯作者：
Takeda K
影响因子：
4.6
作者：
Sun H;Liu X;Li F;Li W;Zhang J;Xiao Z;Shen L;Li Y;Wang F;Yang J
通讯作者：
Yang J