High molecular weight persimmon tannin ameliorates cognition deficits and attenuates oxidative damage in senescent mice induced by D-galactose
High molecular weight persimmon tannin ameliorates cognition deficits and attenuates oxidative damage in senescent mice induced by D-galactose
复制标题
高分子量柿子单宁可改善 D-半乳糖诱导的衰老小鼠认知缺陷并减轻氧化损伤
DOI：
10.1016/j.fct.2011.04.018
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发表时间：
2011-08-01
影响因子：
4.3
通讯作者：
Li, Chun-mei
中科院分区：
文献类型：
--
作者：
Tian, Yan;Zou, Bo;Li, Chun-mei
小鼠皮下注射D-半乳糖（D-gal，150 mg/kg/天）6周，同时给予高分子量柿子缩合单宁（HMWPT）。给药6周后，通过旷场实验和水迷宫实验观察动物行为学变化，并对海马和皮肤进行形态学检查。同时，测定了抗氧化酶活性、非酶抗氧化剂水平以及丙二醛（MDA）含量。结果表明，HMWPT对c-gal所致的小鼠学习记忆障碍有明显的抑制作用。生化检测结果表明，HMWPT显著提高了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性的下降，提高了总抗氧化能力（T-AOC）、谷胱甘肽（GSH）和羟脯氨酸（Hyp）含量的下降（p < 0.01或p < 0.05），并降低升高的单胺氧化酶（MAO）、总胆碱酯酶（TChE）活性和MDA水平D-半乳糖致衰老小鼠血清、肝、脑中的抗氧化活性均显著高于对照组（P < 0.01）。HMWPT还能显著减轻D-半乳糖所致的海马神经元数目减少、神经元变性、核固缩及皮肤表皮和真皮厚度降低（p < 0.01）。(C)2011爱思唯尔有限公司版权所有。
Mice were subcutaneously injected with D-galactose (D-gal, 150mg/kg per day) for 6 weeks and were administered high molecular weight persimmon condensed tannin (HMWPT) simultaneously. After 6 weeks of treatment, the animal behavior was observed in the open field test and water maze test, and the morphology of hippocampus and skin were checked. Meanwhile, the activities of antioxidant enzymes, the levels of non-enzymatic antioxidants, as well as malondialdehyde (MDA) were evaluated. The results indicated that HMWPT markedly inhibited the c-gal induced learning and memory impairment in both open field test and Morris water maze. Biochemical examination revealed that HMWPT significantly increased the decreased activities of superoxide dismutase (SOD), catalase (CAT), elevated the lowered total anti-oxidation capability (T-AOC), glutathione (GSH) and hydroxyproline (Hyp) contents (p < 0.01 or p < 0.05), and decreased the raised monoamine oxidase (MAO), total cholinesterase (TChE) activities and MDA level (p < 0.01) in serum, liver or brain of aging mice induced by D-gal in a dose-dependent fashion. Furthermore, HMWPT significantly and (p < 0.01) attenuated the D-gal induced number decrease, neuronal degeneration and karyopycnosis in cells in the hippocampus and decrease of thickness of skin epidermis and dermis. (C) 2011 Elsevier Ltd. All rights reserved.