Posttranslational Protein Modification in the Salivary Glands of Sjögren's Syndrome Patients.
Posttranslational Protein Modification in the Salivary Glands of Sjögren's Syndrome Patients.
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DOI：
10.1155/2013/548064
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发表时间：
2013
影响因子：
4
通讯作者：
Avalos-Díaz E
中科院分区：
文献类型：
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作者：
Herrera-Esparza R;Rodríguez-Rodríguez M;Pérez-Pérez ME;Badillo-Soto MA;Torres-Del-Muro F;Bollain-Y-Goytia JJ;Pacheco-Tovar D;Avalos-Díaz E
本研究调查了干燥综合征患者唾液腺中的翻译后反应。我们分析了原发性干燥病患者的活检组织，使用免疫组织化学和标签纯化的抗环瓜氨酸蛋白（CCP）抗体检测瓜氨酸肽，并同时评估肽基精氨酸脱亚胺酶2（PAD 2）的存在。目前的工作表明，在一些正常的唾液腺中存在微弱的PAD2酶，虽然PAD2的表达在干燥症患者中显著增加。在干燥病患者的唾液组织中也检测到瓜氨酸化蛋白的存在，这强烈支持在这种情况下蛋白质的原位翻译后修饰。此外，CCP和PAD 2的相互表达表明这种翻译后修饰是酶依赖性的。总之，干燥综合征患者表达了催化机制，产生翻译后反应，可能导致自身抗原触发。
The present study investigated posttranslational reactions in the salivary glands of patients with Sjögren's syndrome. We analysed the biopsies of primary Sjögren's patients using immunohistochemistry and a tag-purified anticyclic citrullinated protein (CCP) antibody to detect citrullinated peptides, and the presence of peptidylarginine deiminase 2 (PAD2) was assessed simultaneously. The present work demonstrated the weak presence of the PAD2 enzyme in some normal salivary glands, although PAD2 expression was increased considerably in Sjögren's patients. The presence of citrullinated proteins was also detected in the salivary tissues of Sjögren's patients, which strongly supports the in situ posttranslational modification of proteins in this setting. Furthermore, the mutual expression of CCP and PAD2 suggests that this posttranslational modification is enzyme dependent. In conclusion, patients with Sjögren's syndrome expressed the catalytic machinery to produce posttranslational reactions that may result in autoantigen triggering.