Biological and immunotoxicity evaluation of antimicrobial peptide-loaded coatings using a layer-by-layer process on titanium.
Biological and immunotoxicity evaluation of antimicrobial peptide-loaded coatings using a layer-by-layer process on titanium.
复制标题
使用钛上的逐层工艺评估抗菌肽负载涂层的生物和免疫毒性。
DOI：
10.1038/srep16336
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发表时间：
2015-11-09
影响因子：
4.6
通讯作者：
Yang G
中科院分区：
文献类型：
--
作者：
Shi J;Liu Y;Wang Y;Zhang J;Zhao S;Yang G
种植体周围炎的预防和控制是种植牙手术中的一个挑战。具有持续抗菌涂层的牙种植体是预防种植体周围炎的理想方法。这项研究报告了在钛表面开发出一种非免疫毒性多层涂层，该涂层具有持续的抗菌活性并限制了早期生物膜的形成。在这项研究中，广谱 AMP Tet213 通过磺基 SMPB 与 IV 型胶原蛋白连接，并已更名为 AMPCol。使用 LBL 技术将多层 AMPCol 涂层组装在光滑的钛表面上。使用 XPS、AFM、接触角分析和 QCM，测量涂层厚度的逐层累积，并确认与对照相比表面润湿性有所增加。观察到 AMPCol 涂层对 HaCaT 无细胞毒性和低红细胞溶血。体内免疫毒性测定表明，腹腔注射 AMPCol 不会影响血清免疫球蛋白水平。这种 AMP 控释涂层可减少革兰氏阳性需氧菌（金黄色葡萄球菌）和革兰氏阴性厌氧菌（牙龈卟啉单胞菌）的生长长达一个月。早期金黄色葡萄球菌生物膜的形成受到涂层的抑制。这种多层涂层具有优异的长期持续抗菌活性，是术前在种植体颈部涂覆以预防种植体周围炎的潜在方法。
The prevention and control of peri-implantitis is a challenge in dental implant surgery. Dental implants with sustained antimicrobial coating are an ideal way of preventing peri-implantitis. This study reports development of a non- immunotoxicity multilayered coating on a titanium surface that had sustained antimicrobial activity and limited early biofilm formation. In this study, the broad spectrum AMP, Tet213, was linked to collagen IV through sulfo-SMPB and has been renamed as AMPCol. The multilayer AMPCol coatings were assembled on smooth titanium surfaces using a LBL technique. Using XPS, AFM, contact angle analysis, and QCM, layer-by-layer accumulation of coating thickness was measured and increased surface wetting compared to controls was confirmed. Non-cytotoxicity to HaCaT and low erythrocyte hemolysis by the AMPCol coatings was observed. In vivo immunotoxicity assays showed IP administration of AMPCol did not effect serum immunoglobulin levels. This coating with controlled release of AMP decreased the growth of both a Gram-positive aerobe (Staphylococcus aureus) and a Gram-negative anaerobe (Porphyromonas gingivalis) up to one month. Early S. aureus biofilm formation was inhibited by the coating. The excellent long-term sustained antimicrobial activity of this multilayer coating is a potential method for preventing peri-implantitis through coated on the neck of implants before surgery.