MOLECULAR AND ANTIGENIC ANALYSES OF SEROTYPES 8 AND 10 OF BOVINE ROTAVIRUSES IN THAILAND
MOLECULAR AND ANTIGENIC ANALYSES OF SEROTYPES 8 AND 10 OF BOVINE ROTAVIRUSES IN THAILAND
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DOI：
10.1099/0022-1317-72-12-2929
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发表时间：
1991-12-01
影响因子：
3.8
通讯作者：
URASAWA, S
中科院分区：
文献类型：
--
作者：
TANIGUCHI, K;URASAWA, T;URASAWA, S
通过交叉中和试验、VP 7基因核苷酸序列测定和RNA-RNA杂交研究了泰国和日本分离的非血清型6牛轮状病毒的抗原性和基因组特性。两种泰国菌株（61 A和A44）与日本分离株KK 3在血清学上相关，后者已被指定为血清型10。相比之下，发现毒株A5与人毒株69 M抗原性相似，具有血清型8特异性，尽管毒株A5与血清型6毒株NCDV显示单向交叉反应。VP 7序列分析证实了这些结果。4个血清型10牛品系（61 A、A44、KK 3和B223）的VP 7基因核苷酸和氨基酸序列高度相似（分别为92.5 ~ 98.2%和96.3 ~ 97.9%）。A5株的VP 7氨基酸序列与血清8型人毒株的相似性分别为91.7%和94.8%（B37和69 M株分别为91.7%和94.8%）。在RNA-RNA杂交实验中，发现三种血清型10牛毒株（61 A、A44和KK 3）之间具有高度的总体相关性，A5和NCDV毒株与三种血清型10病毒也具有中度相关性。本研究中检测的所有牛轮状病毒，无论其血清型特异性如何，均与血清型8的69 M株表现出中度遗传相关性，与血清型2人轮状病毒株的遗传相关性较小。
Antigenic and genomic properties of non-serotype 6 bovine rotaviruses isolated in Thailand and Japan were studied by cross-neutralization tests, nucleotide sequence determination of the VP7 gene, and RNA-RNA hybridization. Two Thai strains (61A and A44) were serologically related to a Japanese isolate KK3 which has been assigned to serotype 10. In contrast, strain A5 was found to be antigenically similar to human strain 69M with serotype 8 specificity, although strain A5 showed a one-way cross-reaction with serotype 6 strain NCDV. VP7 sequence analysis confirmed these results. High degrees of similarity in nucleotide and amino acid sequences (92.5 to 98.2% and 96.3 to 97.9%, respectively) were found among the VP7 genes of the four serotype 10 bovine strains (61A, A44, KK3 and B223). The VP7 amino acid sequence of strain A5 was similar to those of serotype 8 human strains (91.7% and 94.8% for strains B37 and 69M, respectively). In RNA-RNA hybridization experiments, a high level of overall relatedness was found among the three serotype 10 bovine strains (61A, A44 and KK3), and strains A5 and NCDV were also moderately related to the three serotype 10 viruses. All the bovine rotaviruses tested in this study, regardless of their serotype specificity, exhibited a moderate genetic-relatedness to strain 69M of serotype 8, and, to a lesser extent, to serotype 2 human rotavirus strains.