Nucleoporin Nup98 participates in flowering regulation in a CONSTANS-independent mode
Nucleoporin Nup98 participates in flowering regulation in a CONSTANS-independent mode
复制标题
核孔蛋白 Nup98 以不依赖常量的模式参与开花调节。
DOI：
10.1007/s00299-019-02442-w
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发表时间：
2019-10-01
影响因子：
6.2
通讯作者：
Zhang, Xiao-Mei
中科院分区：
文献类型：
--
作者：
Jiang, Shanshan;Xiao, Long;Zhang, Xiao-Mei
两个冗余核孔蛋白基因Nup 98 a和Nup 98 b绕过光周期信号传导中的CO检查点，并从多个途径整合信号，直接靶向FT，用于拟南芥的开花控制。开花调控是植物发育过程中一个重要而广泛研究的事件。尽管核孔蛋白Nup 98已被证明在哺乳动物细胞和酵母的生长和发育中起关键作用，但Nup 98是否参与植物开花控制仍然是未知的。在这项研究中，我们研究了两个Nup 98同源物，Nup 98 a和Nup 98 b，在拟南芥开花调控的功能。结果表明，Nup 98 a和Nup 98 b通过生物钟、光周期和年龄等多种途径冗余抑制开花。nup 98 a和nup 98 b的单突变体与野生型植物相比没有显示出任何明显的异常表型;然而，nup 98 a1 nup 98 b1双突变体显示早花。值得注意的是，Nup 98 a/Nup 98 b以CONSTANS（CO）-独立模式门控开花。因此，Nup 98 a/Nup 98 b绕过光周期信号传导中的CO检查点，并整合来自多个途径的信号，以直接靶向花座T（FT）进行开花控制。此外，我们的研究结果提供了一系列的遗传证据解耦合的开花和衰老的机制在Nup 98 a/Nup 98 b基因在拟南芥，这是经典公认的两个耦合的发育事件。
Key message Two redundant nucleoporin genes Nup98a and Nup98b bypass the CO-check point in photoperiodic signaling and integrated signals from multiple pathways to directly target FT for flowering control in Arabidopsis. Flowering regulation is an important and widely studied plant development event. Even though nucleoporin Nup98 has been proven to play pivotal roles in the growth and development of mammalian cells and yeast, it is still unknown if Nup98 participates in flowering control in plants. In this study, we investigated the function of two Nup98 homologs, Nup98a and Nup98b, in flowering regulation in Arabidopsis. The results showed that Nup98a and Nup98b redundantly inhibit flowering through multiple pathways including clock, photoperiod, and age pathways. Single mutants of nup98a and nup98b do not show any obvious abnormal phenotypes compared to wild-type plants; however, the nup98a1 nup98b1 double mutant displays early flowering. Significantly, Nup98a/Nup98b gate flowering in a CONSTANS (CO)-independent mode. Therefore, Nup98a/Nup98b bypasses the CO checkpoint in photoperiodic signaling and integrated signals from multiple pathways to directly target FLOWERING LOCUS T (FT) for flowering control. In addition, our results provide a line of genetic evidence for uncoupling the mechanism of flowering and senescence at Nup98a/Nup98b genes in Arabidopsis, which are classically recognized as two coupled developmental events.