A clarified position for solanum lycopersicum var. cerasiforme in the evolutionary history of tomatoes (solanaceae)
A clarified position for solanum lycopersicum var. cerasiforme in the evolutionary history of tomatoes (solanaceae)
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DOI：
10.1186/1471-2229-8-130
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发表时间：
2008-12-20
影响因子：
5.3
通讯作者：
Causse, Mathilde
中科院分区：
文献类型：
--
作者：
Ranc, Nicolas;Munos, Stephane;Causse, Mathilde
背景资料：栽培植物种质中存在的自然表型变异性可以使用关联遗传学与分子多态性相关联。然而，有必要考虑种质的遗传结构，以避免错误的关联。了解植物种群的遗传结构有助于推断植物的进化历史。在这种情况下，我们用20个简单的重复序列标记对360个野生、野生和栽培品种进行了基因型分析，并研究了遗传变异的程度和结构。本研究以番茄驯化过程中的红果番茄分支为研究对象，确定了樱桃番茄（Solanum lycopersicum var. cerasiforme）。采用巢式抽样策略建立核心种质，以最少的个体数获得最大的遗传多样性。lycopersicum即驯化的形式。基于模型的分析表明，144 S。番茄cerasiforme种质被分为两组：一组接近驯化组，另一组来自S. lycopersicum和S.茴芹基因组。SSR基因分型也表明，驯化和野生番茄已演变为一个物种复合体与密集水平的杂交。我们编制了基因型和表型数据，以确定子样本的8，24，32和64樱桃番茄加入，捕捉大部分的遗传和形态多样性，目前在整个S。番茄结论：等位基因变异的程度和结构与驯化和现代选择等历史事件有关。并对S.番茄并讨论了cerasiforme在联合遗传学研究中的应用。代表番茄多样性的巢式核心样本在多样性研究中将是有用的。这些核心收藏品的分子和表型变异的定义。这些集合可供科学界使用，并可用作标准化小组，用于协调确定新的有趣基因和更详细地检查驯化过程的努力。
Background: The natural phenotypic variability present in the germplasm of cultivated plants can be linked to molecular polymorphisms using association genetics. However it is necessary to consider the genetic structure of the germplasm used to avoid false association. The knowledge of genetic structure of plant populations can help in inferring plant evolutionary history. In this context, we genotyped 360 wild, feral and cultivated accessions with 20 simple sequence repeat markers and investigated the extent and structure of the genetic variation. The study focused on the red fruited tomato clade involved in the domestication of tomato and confirmed the admixture status of cherry tomatoes ( Solanum lycopersicum var. cerasiforme). We used a nested sample strategy to set-up core collection maximizing the genetic diversity with a minimum of individuals.Results: Molecular diversity was considerably lower in S. lycopersicum i.e. the domesticated form. Model-based analysis showed that the 144 S. lycopersicum var. cerasiforme accessions were structured into two groups: one close to the domesticated group and one resulting from the admixture of the S. lycopersicum and S. pimpinellifolium genomes. SSR genotyping also indicates that domesticated and wild tomatoes have evolved as a species complex with intensive level of hybridization. We compiled genotypic and phenotypic data to identify sub-samples of 8, 24, 32 and 64 cherry tomato accessions that captured most of the genetic and morphological diversity present in the entire S. lycopersicum var. cerasiforme collection.Conclusion: The extent and structure of allelic variation is discussed in relation to historical events like domestication and modern selection. The potential use of the admixed group of S. lycopersicum var. cerasiforme for association genetics studies is also discussed. Nested core collections sampled to represent tomato diversity will be useful in diversity studies. Molecular and phenotypic variability of these core collections is defined. These collections are available for the scientific community and can be used as standardized panels for coordinating efforts on identifying novel interesting genes and on examining the domestication process in more detail.