REGULATORY STUDIES ON THE PROMOTER FOR A GENE GOVERNING SYNTHESIS AND ASSEMBLY OF THE SPORE COAT IN BACILLUS-SUBTILIS
REGULATORY STUDIES ON THE PROMOTER FOR A GENE GOVERNING SYNTHESIS AND ASSEMBLY OF THE SPORE COAT IN BACILLUS-SUBTILIS
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DOI：
10.1016/0022-2836(89)90262-3
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发表时间：
1989-05-20
影响因子：
5.6
通讯作者：
LOSICK, R
中科院分区：
文献类型：
--
作者：
CUTTING, S;PANZER, S;LOSICK, R
Gere是枯草芽孢杆菌的一个调节基因，它控制着孢子壳的合成和组装，是产生抗溶菌酶和精通萌发的孢子所必需的。我们报道了Gere启动子的鉴定及其表达调控的研究。我们发现Gere在孢子形成的第四个小时(阶段V)被激活，并且这种表达仅限于孢子囊的母细胞室。在36个不同发育突变体中测量了Gere表达水平的相关性研究表明，Gere的有效表达需要几乎所有测试的spo0-IV基因以及某些spoV基因的产物。根据其诱导的时间、表达的区区化和对其他SPO基因的依赖模式，推测Gere与先前研究的孢子壳蛋白基因ctoA协同调控。Gera和Cota可能是一个发育调节基因的成员，其产物与孢子壳的组装有关。
gerE is a regulatory gene of Bacillus subtilis that governs the synthesis and assembly of the spore coat and is required for the production of spores that are lysozyme-resistant and germination-proficient. We report the identification of the promoter for gerE and studies on the regulation of its expression. We show that gerE is switched on at the fourth hour of sporulation (stage-V) and that this expression is restricted to the mother-cell chamber of the sporangium. Dependency studies in which the level of gerE expression was measured in 36 different developmental mutants indicate that efficient expression of gerE requires the products of almost all spo0-IV genes tested as well as certain spoV genes. On the basis of its time of induction, compartmentalization of expression and pattern of dependence on other spo genes, gerE is inferred to be regulated co-ordinately with the previously studied spore coat protein gene ctoA. gerA and cotA may be members of a developmental regulon of genes whose products are involved in the assembly of the spore coat.