Bioaugmentation of activated sludge towards 3-chloroaniline removal with a mixed bacterial population carrying a degradative plasmid.
Bioaugmentation of activated sludge towards 3-chloroaniline removal with a mixed bacterial population carrying a degradative plasmid.
复制标题
DOI：
10.1016/j.biortech.2009.01.060
复制
发表时间：
2009-06
影响因子：
11.4
通讯作者：
S. Bathe;N. Schwarzenbeck;M. Hausner
中科院分区：
文献类型：
--
作者：
S. Bathe;N. Schwarzenbeck;M. Hausner
采用生物强化技术，在连续运行的活性污泥反应器中对3-氯苯胺（3CA）进行降解。在第一步中，将携带降解质粒pNB 2的3CA降解睾丸酮丛毛单胞菌菌株添加到生物膜反应器中，实现了3CA的完全降解以及质粒在固有生物膜群体中的传播。然后用从载体材料中除去的生物膜细胞的悬浮液或用含生物膜的载体材料对第二组反应器进行生物扩增。3CA降解迅速建立在所有的生物强化反应器，随后由非生物强化控制反应器的缓慢适应。响应于3CA浓度的变化，所有反应器都表现出暂时的性能故障。而重复的控制反应器偏离他们的行为，生物增强反应器出现在他们的性能和人口动态更重现。最后，载体生物强化反应器表现出了改善的性能，在高3CA进水浓度的悬浮生物强化反应器的存在。相比之下，在一个控制反应器中的降解完全失败，但在剩余的控制反应器中迅速建立。
A bioaugmentation approach combining several strategies was applied to achieve degradation of 3-chloroaniline (3CA) in semicontinuous activated sludge reactors. In a first step, a 3CA-degrading Comamonas testosteroni strain carrying the degradative plasmid pNB2 was added to a biofilm reactor, and complete 3CA degradation together with spread of the plasmid within the indigenous biofilm population was achieved. A second set of reactors was then bioaugmented with either a suspension of biofilm cells removed from the carrier material or with biofilm-containing carrier material. 3CA degradation was established rapidly in all bioaugmented reactors, followed by a slow adaptation of the non-bioaugmented control reactors. In response to variations in 3CA concentration, all reactors exhibited temporary performance breakdowns. Whereas duplicates of the control reactors deviated in their behaviour, the bioaugmented reactors appeared more reproducible in their performance and population dynamics. Finally, the carrier-bioaugmented reactors showed an improved performance in the presence of high 3CA influent concentrations over the suspension-bioaugmented reactors. In contrast, degradation in one control reactor failed completely, but was rapidly established in the remaining control reactor.